PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST HUMAN PROSTATE SPECIFIC ANTIGEN

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BAYAT ALI AHMAD; GHODS ROYA; SHABANI MAHDI; MAHMOUDI AHMAD REZA; YEGANEH OMID; HASSANNIA HADI; SADEGHITABAR ALI; BALAY GOLI LEILA; NOUTASH HAGHIGHAT FARZANEH; SARRAFZADEH ALI REZA; JEDDI TEHRANI MAHMOOD

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Journal: Avicenna Journal of Medical Biotechnology Year:2015 | Volume:7 | Issue:1 Page(s): 2-7

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Title

PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST HUMAN PROSTATE SPECIFIC ANTIGEN

Author(s)

Keywords

ELISA

IMMUNOHISTOCHEMISTRY

MONOCLONAL ANTIBODY

PROSTATE SPECIFIC ANTIGEN

WESTERN BLOT

Abstract

Background: PROSTATE SPECIFIC ANTIGEN (PSA) is an important laboratory marker for diagnosis of prostatic cancer. Thus, development of diagnostic tools specific for PSA plays an important role in screening, monitoring and early diagnosis of prostate cancer. In this paper, the production and characterization of a panel of murine monoclonal antibodies (mAbs) against PSA have been presented.Methods: Balb/c mice were immunized with PSA, which was purified from seminal plasma. Splenocytes of hyperimmunized mice were extracted and fused with Sp2/0 cells. By adding selective HAT medium, hybridoma cells were established and positive clones were selected by ELISA after four times of cloning. The isotypes of produced mAbs were determined by ELISA and then purified from ascitic fluids using Hi-Trap protein G column. The reactivities of the mAbs were examined with the purified PSA and seminal plasma by ELISA and WESTERN BLOT techniques. Furthermore, the reactivities of the mAbs were assessed in Prostate Cancer (PCa), Benign Prostatic Hyperplasia (BPH) and brain cancer tissues by IMMUNOHISTOCHEMISTRY (IHC).Results: Five anti-PSA mAbs (clones: 2G2-B2, 2F9-F4, 2D6-E8, IgG1/K) and clones (2C8-E9, 2G3-E2, IgG2a/K) were produced and characterized. All mAbs, except 2F9- F4 detected the expression of PSA in PCa and BPH tissues and none of them reacted with PSA in brain cancer tissue in IHC. Besides, all mAbs could detect a protein band around 33 kDa in human seminal plasma in WESTERN BLOT.Conclusion: These mAbs can specifically recognize PSA and may serve as a component of PSA diagnostic kit in various biological fluids.

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APA: Copy

BAYAT, ALI AHMAD, GHODS, ROYA, SHABANI, MAHDI, MAHMOUDI, AHMAD REZA, YEGANEH, OMID, HASSANNIA, HADI, SADEGHITABAR, ALI, BALAY GOLI, LEILA, NOUTASH HAGHIGHAT, FARZANEH, SARRAFZADEH, ALI REZA, & JEDDI TEHRANI, MAHMOOD. (2015). PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST HUMAN PROSTATE SPECIFIC ANTIGEN. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 7(1), 2-7. SID. https://sid.ir/paper/313861/en

Vancouver: Copy

BAYAT ALI AHMAD, GHODS ROYA, SHABANI MAHDI, MAHMOUDI AHMAD REZA, YEGANEH OMID, HASSANNIA HADI, SADEGHITABAR ALI, BALAY GOLI LEILA, NOUTASH HAGHIGHAT FARZANEH, SARRAFZADEH ALI REZA, JEDDI TEHRANI MAHMOOD. PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST HUMAN PROSTATE SPECIFIC ANTIGEN. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2015;7(1):2-7. Available from: https://sid.ir/paper/313861/en

IEEE: Copy

ALI AHMAD BAYAT, ROYA GHODS, MAHDI SHABANI, AHMAD REZA MAHMOUDI, OMID YEGANEH, HADI HASSANNIA, ALI SADEGHITABAR, LEILA BALAY GOLI, FARZANEH NOUTASH HAGHIGHAT, ALI REZA SARRAFZADEH, and MAHMOOD JEDDI TEHRANI, “PRODUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST HUMAN PROSTATE SPECIFIC ANTIGEN,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 7, no. 1, pp. 2–7, 2015, [Online]. Available: https://sid.ir/paper/313861/en

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