THE POTENTIAL OF BRITTLE STAR STAR EXTRACTED EXTRACTED POLYSACCHARIDE POLYSACCHARIDE PRO-MOTE IN PROMOTING APOPTOSIS APOPTOSIS VIA INTRINSIC INTRINSIC SIGNALING SIGNALING PATHWAY-PATHWAY

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BAHARARA JAVAD; AMINI ELAHEH

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Journal Paper

Paper Information

Journal: Avicenna Journal of Medical Biotechnology Year:2015 | Volume:7 | Issue:4 (27) Page(s): 151-158

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Title

THE POTENTIAL OF BRITTLE STAR STAR EXTRACTED EXTRACTED POLYSACCHARIDE POLYSACCHARIDE PRO-MOTE IN PROMOTING APOPTOSIS APOPTOSIS VIA INTRINSIC INTRINSIC SIGNALING SIGNALING PATHWAY-PATHWAY

Author(s)

BAHARARA JAVAD | AMINI ELAHEH | Issue Writer Certificate

Keywords

APOPTOSIS

BRITTLE STAR

HELA CELLS

POLYSACCHARIDES

Abstract

Background: Anti-cancer potential of marine natural products such as POLYSACCHARIDES represented therapeutic potential in oncological researches. In this study, we extracted total polysaccharide from BRITTLE STAR [Ophiocoma erinaceus (O. erinaceus)] was extracted and investigated the chemopreventive efficacy of Persian Gulf BRITTLE STAR polysaccharide was investigated in HeLa human cervical cancer cells.Methods: To extract polysaccharide, dried BRITTLE STARs were grinded ground and ex-tracted mechanically. Then, detection of polysaccharide was performed by phenol sulfuric acid, Ultra Violet (UV)-sulfuric acid method and FTIR. The anti proliferative activity of isolated polysaccharide was examined by MTT assay and evaluation of cell death induced was done through morphological cell changes; Propodium Iodide staining, fluorescence microscopy and caspase-3, -9 enzymatic measurements. To assess its un-derlying mechanism, expression of Bax, Bcl-2 was evaluated.Results: The polysaccharide detection methods demonstrated isolation of crude poly-saccharide from Persian Gulf BRITTLE STAR. The results revealed that O. erinaceus poly-saccharide suppressed the proliferation of HELA CELLS in a dose and time dependent manner. Morphological observation of DAPI and Acridine Orange/Propodium Iodide staining was documented by typical characteristics of APOPTOSIS apoptotic cell death. Flow cytometry analyses exhibited the accumulation of treated cells in sub-G1 region. Additionally, polysaccharide extracted induced intrinsic APOPTOSIS via up-regulation of caspase-3, caspase-9 and Bax along with down-regulation of Bcl-2 in HELA CELLS.Conclusion: Taken together, the APOPTOSIS inducing effect of BRITTLE STAR polysaccharide via intrinsic pathway confirmed the anti tumor potential of marine polysaccharide. Therefore, these findings proposed new insight into anti cancer properties of BRITTLE STAR polysaccharide as a promising agent in cervical cancer treatment.

Cites

The p53 Modulated Cytotoxicity of Ophiocoma scolopendrina Polysaccharide Against Resistance Ovarian Cancer Cells

References

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APA: Copy

BAHARARA, JAVAD, & AMINI, ELAHEH. (2015). THE POTENTIAL OF BRITTLE STAR STAR EXTRACTED EXTRACTED POLYSACCHARIDE POLYSACCHARIDE PRO-MOTE IN PROMOTING APOPTOSIS APOPTOSIS VIA INTRINSIC INTRINSIC SIGNALING SIGNALING PATHWAY-PATHWAY. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 7(4 (27)), 151-158. SID. https://sid.ir/paper/313883/en

Vancouver: Copy

BAHARARA JAVAD, AMINI ELAHEH. THE POTENTIAL OF BRITTLE STAR STAR EXTRACTED EXTRACTED POLYSACCHARIDE POLYSACCHARIDE PRO-MOTE IN PROMOTING APOPTOSIS APOPTOSIS VIA INTRINSIC INTRINSIC SIGNALING SIGNALING PATHWAY-PATHWAY. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2015;7(4 (27)):151-158. Available from: https://sid.ir/paper/313883/en

IEEE: Copy

JAVAD BAHARARA, and ELAHEH AMINI, “THE POTENTIAL OF BRITTLE STAR STAR EXTRACTED EXTRACTED POLYSACCHARIDE POLYSACCHARIDE PRO-MOTE IN PROMOTING APOPTOSIS APOPTOSIS VIA INTRINSIC INTRINSIC SIGNALING SIGNALING PATHWAY-PATHWAY,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 7, no. 4 (27), pp. 151–158, 2015, [Online]. Available: https://sid.ir/paper/313883/en

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