EVALUATION OF EFFLUX PUMPS GENE EXPRESSION IN RESISTANT PSEUDOMONAS AERUGINOSA ISOLATES IN AN IRANIAN REFERRAL HOSPITAL

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

POURAKBARI BABAK; YASLIANIFARD SAHAR; YASLIANIFARD SOMAYEH; MAHMOUDI SHIMA; KESHAVARZ VALIAN SEPIDEH; MAMISHI SETARE

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Microbiology Year:2016 | Volume:8 | Issue:4 Page(s): 0-0

Download Full-Text

View:

281

Download:

122

Cites:

Information Journal Paper

Title

EVALUATION OF EFFLUX PUMPS GENE EXPRESSION IN RESISTANT PSEUDOMONAS AERUGINOSA ISOLATES IN AN IRANIAN REFERRAL HOSPITAL

Author(s)

Keywords

PSEUDOMONAS AERUGINOSA

EFFLUX PUMP

REAL-TIME PCR

Abstract

Background and Objectives: PSEUDOMONAS AERUGINOSA (PA) is one of the most important causes of nosocomial infectionsand has an intrinsic resistance to many antibiotics. Among all the resistance-nodulation-division (RND) pumps of P. aeruginosa, MexAB-OprM is the first EFFLUX PUMP found to target multiple classes of antibiotics. This study was aimed to evaluatethe expression level of genes expressing MexAB-OprM in clinical isolates of P. aeruginosa.Materials and Methods: In this study, 45 P. aeruginosa strains were isolated from patients admitted to Children's MedicalCenter Hospital, an Iranian referral hospital. Disk diffusion and Minimum Inhibitory Concentration (MIC) methods wereused for determination of the patterns of resistance to antibiotics. REAL-TIME PCR was used to investigate the expression levelof genes of MexAB-OprM EFFLUX PUMP.Results: Among 45 resistant PA isolates, the frequency of genes overexpression was as follows: MexA (n=25, 55.5%), MexB(n=24, 53.3%) and OprM (n=16, 35.5%). In addition, in 28 strains (62%) overexpression was observed in one of the studiedthree genes of MexAB-OprM EFFLUX PUMP.Conclusion: In our study 28 isolates (62%) had increased expression level of EFFLUX PUMPs genes, MexAB-OprM. Althoughthe EFFLUX PUMPs play important roles in increasing the resistance towards different antibiotics but the role of other agentsand mechanisms in evolution of resistance should not be ignored. Since the concomitant overproduction of other Mex effluxsystems might have additive effects on antibiotic resistance, the co-expressing of a multicomponent EFFLUX PUMP is recommended.On the other hand, the concomitant overproduction of two Mex pumps might have additive effects on resistance toantibiotic. Therefore co-expressing of Mex efflux systems is recommended.

Cites

THE EFFECT OF SILYBIN ENCAPSULATED IN NANOPARTICLES ON OPRM GENE EXPRESSION IN DRUG RESISTANT ISOLATES OF PSEUDOMONAS AERUGINOSA

References

No record.

Cite

APA: Copy

POURAKBARI, BABAK, YASLIANIFARD, SAHAR, YASLIANIFARD, SOMAYEH, MAHMOUDI, SHIMA, KESHAVARZ VALIAN, SEPIDEH, & MAMISHI, SETARE. (2016). EVALUATION OF EFFLUX PUMPS GENE EXPRESSION IN RESISTANT PSEUDOMONAS AERUGINOSA ISOLATES IN AN IRANIAN REFERRAL HOSPITAL. IRANIAN JOURNAL OF MICROBIOLOGY, 8(4), 0-0. SID. https://sid.ir/paper/315403/en

Vancouver: Copy

POURAKBARI BABAK, YASLIANIFARD SAHAR, YASLIANIFARD SOMAYEH, MAHMOUDI SHIMA, KESHAVARZ VALIAN SEPIDEH, MAMISHI SETARE. EVALUATION OF EFFLUX PUMPS GENE EXPRESSION IN RESISTANT PSEUDOMONAS AERUGINOSA ISOLATES IN AN IRANIAN REFERRAL HOSPITAL. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2016;8(4):0-0. Available from: https://sid.ir/paper/315403/en

IEEE: Copy

BABAK POURAKBARI, SAHAR YASLIANIFARD, SOMAYEH YASLIANIFARD, SHIMA MAHMOUDI, SEPIDEH KESHAVARZ VALIAN, and SETARE MAMISHI, “EVALUATION OF EFFLUX PUMPS GENE EXPRESSION IN RESISTANT PSEUDOMONAS AERUGINOSA ISOLATES IN AN IRANIAN REFERRAL HOSPITAL,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 8, no. 4, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/315403/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops