THE EFFECT OF SILYBIN ENCAPSULATED IN NANOPARTICLES ON OPRM GENE EXPRESSION IN DRUG RESISTANT ISOLATES OF PSEUDOMONAS AERUGINOSA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOHAMMADIPOUR AREF; RANJI NAJMEH; ASADPOUR LEILA

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ) Year:2017 | Volume:20 | Issue:5 (122) Page(s): 79-88

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

845

Download:

Cites:

Information Journal Paper

Title

THE EFFECT OF SILYBIN ENCAPSULATED IN NANOPARTICLES ON OPRM GENE EXPRESSION IN DRUG RESISTANT ISOLATES OF PSEUDOMONAS AERUGINOSA

Author(s)

MOHAMMADIPOUR AREF | RANJI NAJMEH | ASADPOUR LEILA | Issue Writer Certificate

Keywords

CIPROFLOXACINQ2

NANOPARTICLESQ1

OPRMQ1

PSEUDOMONAS AERUGINOSAQ1

QUANTITATIVE REAL-TIME PCRQ1

SILYBINQ1

Abstract

Background: PSEUDOMONAS AERUGINOSAis an opportunistic nosocomial pathogen that using several classes of antibiotics to treat has been led to the emergence of multiple drug resistance. One of the drug resistance mechanisms inPseudomonas aeruginosa is overexpression of mexXY-oprM efflux pump system. SILYBIN as main flavonolignan of silymarin extracted from Silybum marianum is a hepatoprotective agent that its anti-bacterial properties was studied, recently. In this study, the effect of combination of SILYBIN and CIPROFLOXACIN onoprM gene expression in clinical isolates of PSEUDOMONAS AERUGINOSA was evaluated.Materials and Methods: In this study, seven CIPROFLOXACIN resistant isolates of PSEUDOMONAS AERUGINOSAwere treated by CIPROFLOXACIN (1.2MIC) only (control sample) and in the combination with SILYBIN-encapsulated micelle (nanoparticles) (test sample). After 24h, RNA extraction and cDNA synthesis were performed in SILYBIN treated and un-treated cells and OPRMgene expression was quantitatively investigated by real time PCR method.Results: Results of this study showed that a SILYBIN encapsulated in NANOPARTICLES (400mg/ml) induces death up to 50% in resistant isolates treated by CIPROFLOXACIN (1.2MIC) during 24h. Also, QUANTITATIVE REAL-TIME PCR analysis revealed that SILYBIN encapsulated in NANOPARTICLES decreases the expression of opr M gene compared to SILYBIN untreated cells.Conclusion: It seems that Decrease of OPRM expression in resistant isolates lead to decrease of mexAB-opr M and mexXY-oprM in cell surface, subsequently decrease of antibiotic withdrawal to extracellular environment and increase of sensitivity to antibiotics.

Cites

Isolation of TEM beta-lactamase gene in Pseudomonas aeruginosa and Imipenem Effect on Expression of TEM Gene by Real-Time PCR from Burn Wound Samples

References

Cite

APA: Copy

MOHAMMADIPOUR, AREF, RANJI, NAJMEH, & ASADPOUR, LEILA. (2017). THE EFFECT OF SILYBIN ENCAPSULATED IN NANOPARTICLES ON OPRM GENE EXPRESSION IN DRUG RESISTANT ISOLATES OF PSEUDOMONAS AERUGINOSA. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 20(5 (122)), 79-88. SID. https://sid.ir/paper/69295/en

Vancouver: Copy

MOHAMMADIPOUR AREF, RANJI NAJMEH, ASADPOUR LEILA. THE EFFECT OF SILYBIN ENCAPSULATED IN NANOPARTICLES ON OPRM GENE EXPRESSION IN DRUG RESISTANT ISOLATES OF PSEUDOMONAS AERUGINOSA. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2017;20(5 (122)):79-88. Available from: https://sid.ir/paper/69295/en

IEEE: Copy

AREF MOHAMMADIPOUR, NAJMEH RANJI, and LEILA ASADPOUR, “THE EFFECT OF SILYBIN ENCAPSULATED IN NANOPARTICLES ON OPRM GENE EXPRESSION IN DRUG RESISTANT ISOLATES OF PSEUDOMONAS AERUGINOSA,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 20, no. 5 (122), pp. 79–88, 2017, [Online]. Available: https://sid.ir/paper/69295/en

Related Journal Papers