Effectiveness of an inactivated paratuberculosis vaccine in Iranian sheep flocks using the Mycobacterium avium subsp paratuberculosis 316F strain

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KESHAVARZ ROUHOLAH; MOSAVARI NADER; TADAYON KEYVAN; HAGHKHAH MASOUD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Microbiology Year:2018 | Volume:10 | Issue:2 Page(s): 117-122

Download Full-Text

View:

236

Download:

104

Cites:

Information Journal Paper

Title

Effectiveness of an inactivated paratuberculosis vaccine in Iranian sheep flocks using the Mycobacterium avium subsp paratuberculosis 316F strain

Author(s)

KESHAVARZ ROUHOLAH | MOSAVARI NADER | TADAYON KEYVAN | HAGHKHAH MASOUD | Issue Writer Certificate

Keywords

Mycobacterium avium subspecies paratuberculosis

John's disease

IS900

F57

Vaccine

Abstract

Background and Objectives: Paratuberculosis (PTb) (John's disease) is an incurable chronic intestinal infection that mainly affects ruminants. PTb is caused by Mycobacterium avium subspecies paratuberculosis (MAP) with a global distribution. Despite evidences on MAP contribution in Crohn's disease its causal role is still a matter of controversy. In ruminant farming, vaccination is broadly accepted as an effective control measure of PTb. This article describes preparation and field trial of an inactivated PTb Vaccine made from the MAP 316F strain. Materials and Methods: Formulation of the Vaccine was conducted based on the method traditionally used in the UK. Identity of the MAP strain was authenticated by PCR-IS900 and PCR-F57 tests. In the field, a group of 100 lambs (3-8 weeks old) were subcutaneously inoculated with the Vaccine preparation under study. These animals, pre-vaccination, were all PTb ELISA negative. Serum level of antibody was determined by ELISA on days 0, 30, 60, 120 and 240, postvaccination. Results: In PCR-900 and PCR-F57, the MAP 316F strain produced two fragments of 560 and 704 bp length respectively, a confirmation of its identity as MAP bacterium. In the field trial and at the arranged time intervals, the achieved blood serum levels of antibody, attributable to the Vaccine formulation, displayed considerably high values. Conclusion: Given that the PTb-caused economical losses in the Iranian environment are dramatically high and also the fact that future of state policy on control of PTb remains unknown, we belive vaccination of animals is the best recommendable practice.

Multimedia

No record.

Cites

No record.

References

No record.

Cite

APA: Copy

KESHAVARZ, ROUHOLAH, MOSAVARI, NADER, TADAYON, KEYVAN, & HAGHKHAH, MASOUD. (2018). Effectiveness of an inactivated paratuberculosis vaccine in Iranian sheep flocks using the Mycobacterium avium subsp paratuberculosis 316F strain. IRANIAN JOURNAL OF MICROBIOLOGY, 10(2), 117-122. SID. https://sid.ir/paper/315465/en

Vancouver: Copy

KESHAVARZ ROUHOLAH, MOSAVARI NADER, TADAYON KEYVAN, HAGHKHAH MASOUD. Effectiveness of an inactivated paratuberculosis vaccine in Iranian sheep flocks using the Mycobacterium avium subsp paratuberculosis 316F strain. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2018;10(2):117-122. Available from: https://sid.ir/paper/315465/en

IEEE: Copy

ROUHOLAH KESHAVARZ, NADER MOSAVARI, KEYVAN TADAYON, and MASOUD HAGHKHAH, “Effectiveness of an inactivated paratuberculosis vaccine in Iranian sheep flocks using the Mycobacterium avium subsp paratuberculosis 316F strain,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 10, no. 2, pp. 117–122, 2018, [Online]. Available: https://sid.ir/paper/315465/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops