DEVELOPMENT OF RT-PCR USING EXTERNAL AND INTERNAL POSITIVE CONTROLS BASED ON 5' UNTRANSLATED REGION (UTR) FOR MOLECULAR DETECTION OF AVIAN INFECTIOUS BRONCHITIS VIRUS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MAHDI A.; GHALYANCHI LANGEROUDI A.; HOSSEINI S.M.; SOLEIMANI M.; MOHSENI A.H.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF VIROLOGY Year:2014 | Volume:8 | Issue:4 Page(s): 25-33

Download Full-Text

View:

522

Download:

187

Cites:

Information Journal Paper

Title

DEVELOPMENT OF RT-PCR USING EXTERNAL AND INTERNAL POSITIVE CONTROLS BASED ON 5' UNTRANSLATED REGION (UTR) FOR MOLECULAR DETECTION OF AVIAN INFECTIOUS BRONCHITIS VIRUS

Author(s)

Keywords

AVIAN INFECTIOUS BRONCHITIS VIRUS

INTERNAL POSITIVE CONTROL

DETECTION

RT-PCR

Abstract

Background and Aims: Infectious bronchitis virus (IBV) belongs to the group of gamma coronaviruses along with other avian coronaviruses. The disease caused by IBV can appear similar to infectious laryngotracheitis, avian influenza, and velogenic Newcastle disease, which are high priority diseases. The clinical signs can be accompanied by mortalities in broiler chickens and reduced eggshell and albumin quality in layer hens, leading to economic loss for the poultry industry. Rapid DETECTION of IBV is useful for implementation of control measures, research purposes, and understanding the epidemiology and evolution of IBVs. The aim of the present study was the rapid identification of IB with the molecular method, which targets the 5’ untranslated region (UTR) gene of IBV that is less variable than the other genes, with homologies greater than 90% among IBV strains.Materials and Methods: The primers designed to amplify a conserved fragment of the gene. Analytical sensitivity and specificity of the assay were determined.Results: The results of specificity exhibited the specific amplification of the designed primers for IBV. Sensitivity was 10 pg/ml of the pTZ57R/T-5' UTR. This is the first report of RT-PCR method coupled with construction of comparative INTERNAL POSITIVE CONTROL (IPC) according to 5´UTR gene for accurate DETECTION of IBV. 100 fg/ml of the IPC amplified in the presence of the limit of DETECTION (10 pg/ml) of 5' UTR gene was determined as the optimal concentration of IPC plasmid for RT-PCR of clinical specimens.Conclusion The RT-PCR assay presented provides a time saving, sensitive, and reliable method for DETECTION of IBV.

Cites

No record.

References

No record.

Cite

APA: Copy

MAHDI, A., GHALYANCHI LANGEROUDI, A., HOSSEINI, S.M., SOLEIMANI, M., & MOHSENI, A.H.. (2014). DEVELOPMENT OF RT-PCR USING EXTERNAL AND INTERNAL POSITIVE CONTROLS BASED ON 5' UNTRANSLATED REGION (UTR) FOR MOLECULAR DETECTION OF AVIAN INFECTIOUS BRONCHITIS VIRUS. IRANIAN JOURNAL OF VIROLOGY, 8(4), 25-33. SID. https://sid.ir/paper/315705/en

Vancouver: Copy

MAHDI A., GHALYANCHI LANGEROUDI A., HOSSEINI S.M., SOLEIMANI M., MOHSENI A.H.. DEVELOPMENT OF RT-PCR USING EXTERNAL AND INTERNAL POSITIVE CONTROLS BASED ON 5' UNTRANSLATED REGION (UTR) FOR MOLECULAR DETECTION OF AVIAN INFECTIOUS BRONCHITIS VIRUS. IRANIAN JOURNAL OF VIROLOGY[Internet]. 2014;8(4):25-33. Available from: https://sid.ir/paper/315705/en

IEEE: Copy

A. MAHDI, A. GHALYANCHI LANGEROUDI, S.M. HOSSEINI, M. SOLEIMANI, and A.H. MOHSENI, “DEVELOPMENT OF RT-PCR USING EXTERNAL AND INTERNAL POSITIVE CONTROLS BASED ON 5' UNTRANSLATED REGION (UTR) FOR MOLECULAR DETECTION OF AVIAN INFECTIOUS BRONCHITIS VIRUS,” IRANIAN JOURNAL OF VIROLOGY, vol. 8, no. 4, pp. 25–33, 2014, [Online]. Available: https://sid.ir/paper/315705/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops