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Title

CHARACTERIZATION OF AMPC, CTX-M AND MBLS TYPES OF β-LACTAMASES IN CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE AND ESCHERICHIA COLI PRODUCING EXTENDED SPECTRUM β-LACTAMASES IN KERMAN, IRAN

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Abstract

 Background: Extended spectrum β-lactamases (ESBLs) and AmpC b-lactamases enzyme are major sources of resistance to b-lactam antibiotics especially in Enterobacteriaceae such as ESCHERICHIA COLI and KLEBSIELLA PNEUMONIAe. Increasing frequency of the co-existence of ESBLS with AmpC- b-lactamases in bacteria is a serious threat for treating bacterial infections.Objectives: The aim of this study was to determine the presence of AmpC and CTX-M types of b-lactamases in clinical isolates of E. coli and K. pneumoniae producing ESBLS.Materials and Methods: Resistance to different antibiotics was determined using the standard disk diffusion method. ESBLS, MBLs and AmpC- b-lactamases were detected by the combination double disk test (CDDT) method and polymerase chain reaction (PCR) was used to determine blaCTX-M genes in the ESBLS and AmpC positive isolates.Results: The prevalence of ESBLS and AmpC- b-lactamase producer isolates was 181 (43.8%) and 133 (37.2%), respectively. The prevalence of blaCTX-M among isolates was 61 (14.7%).Conclusions: Outbreak of isolates co-expressing AmpC- b-lactamases and ESBLS can cause serious problems in the future, regarding the treatment of infections caused by these common enteric pathogens.

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    APA: Copy

    MANSOURI, SHAHLA, KALANTAR NEYESTANAKI, DAVOOD, SHOKOOHI, MOSTAFA, HALIMI, SHAHNAZ, BEIGVERDI, REZA, REZAGHOLEZADEH, FERESHTEH, & HASHEMI, ALI. (2014). CHARACTERIZATION OF AMPC, CTX-M AND MBLS TYPES OF β-LACTAMASES IN CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE AND ESCHERICHIA COLI PRODUCING EXTENDED SPECTRUM β-LACTAMASES IN KERMAN, IRAN. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 7(2), 0-0. SID. https://sid.ir/paper/317353/en

    Vancouver: Copy

    MANSOURI SHAHLA, KALANTAR NEYESTANAKI DAVOOD, SHOKOOHI MOSTAFA, HALIMI SHAHNAZ, BEIGVERDI REZA, REZAGHOLEZADEH FERESHTEH, HASHEMI ALI. CHARACTERIZATION OF AMPC, CTX-M AND MBLS TYPES OF β-LACTAMASES IN CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE AND ESCHERICHIA COLI PRODUCING EXTENDED SPECTRUM β-LACTAMASES IN KERMAN, IRAN. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2014;7(2):0-0. Available from: https://sid.ir/paper/317353/en

    IEEE: Copy

    SHAHLA MANSOURI, DAVOOD KALANTAR NEYESTANAKI, MOSTAFA SHOKOOHI, SHAHNAZ HALIMI, REZA BEIGVERDI, FERESHTEH REZAGHOLEZADEH, and ALI HASHEMI, “CHARACTERIZATION OF AMPC, CTX-M AND MBLS TYPES OF β-LACTAMASES IN CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE AND ESCHERICHIA COLI PRODUCING EXTENDED SPECTRUM β-LACTAMASES IN KERMAN, IRAN,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 7, no. 2, pp. 0–0, 2014, [Online]. Available: https://sid.ir/paper/317353/en

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