ASSOCIATION OF HP1181 AND HP1184 GENES WITH THE ACTIVE EFFLUX PHENOTYPE IN MULTIDRUG-RESISTANT ISOLATES OF HELICOBACTER PYLORI

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FALSAFI TAHEREH; EHSANI AZADEH; ATTARAN BAHAREH; NIKNAM VAHID

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Journal: Jundishapur Journal of Microbiology Year:2016 | Volume:9 | Issue:4 Page(s): 0-0

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Title

ASSOCIATION OF HP1181 AND HP1184 GENES WITH THE ACTIVE EFFLUX PHENOTYPE IN MULTIDRUG-RESISTANT ISOLATES OF HELICOBACTER PYLORI

Author(s)

FALSAFI TAHEREH | EHSANI AZADEH | ATTARAN BAHAREH | NIKNAM VAHID | Issue Writer Certificate

Keywords

EFFLUX

MULTIDRUG-RESISTANT

HP1184

HP1181

HELICOBACTER PYLORI

Abstract

Background: During the last decades the rate of multidrug resistance among clinical HELICOBACTER PYLORI isolates has increased. Active pumping out of the drugs may be an important mechanism for multidrug resistance in H. pylori strains.Objectives: The aim of this study was to evaluate the association of two H. pylori EFFLUX-genes, HP1181 and HP1184 with the active-EFFLUX phenotype in MDR clinical-strains of H. pylori.Materials and Methods: Minimal inhibitory concentration (MIC) and drug accumulation for b-lactames, Tetracycline (TET), Erythromycin (ERY), Metronidazole (MTZ), Ciprofloxacin (CIP) and Ethidium Bromide (EtBr) was performed in the presence and absence of carbonyl cyanide M-Chlorophenyl Hydrazone (CCCP). Presence of HP1181 and HP1184 genes was detected by the polymerase chain reaction (PCR). Real Time (RT)-PCR was performed to compare expression of EFFLUX genes by MDR strains, demonstrating active EFFLUX with the strains without active EFFLUX.Results: Two- to four-fold decrease in minimum inhibitory concentration (MIC) and two-fold increase in accumulation were observed for EtBr in the presence of CCCP for 67% (8) of 12 MDR strains. With CCCP, two- to four-fold decrease in MIC and 1.4- to 1.8-fold increase in the accumulation of b-lactames, TET, CIP and MTZ were obtained for 42% (5) of the MDR strains. Six, five and three of the 12 MDR strains amplified HP1184, HP1181, and both of them, respectively. The RT-PCR product for expression of HP1181 by MDR strains was approximately 100 bp shorter than that of the 26695 susceptible standard strain.Conclusions: Expression of the genes HP1184 and HP1181 are associated with the specific active EFFLUX of EtBr and non-related antibiotics, respectively. For displaying these phenotypes, a post-transcriptional regulation step may be required.

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APA: Copy

FALSAFI, TAHEREH, EHSANI, AZADEH, ATTARAN, BAHAREH, & NIKNAM, VAHID. (2016). ASSOCIATION OF HP1181 AND HP1184 GENES WITH THE ACTIVE EFFLUX PHENOTYPE IN MULTIDRUG-RESISTANT ISOLATES OF HELICOBACTER PYLORI. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 9(4), 0-0. SID. https://sid.ir/paper/317604/en

Vancouver: Copy

FALSAFI TAHEREH, EHSANI AZADEH, ATTARAN BAHAREH, NIKNAM VAHID. ASSOCIATION OF HP1181 AND HP1184 GENES WITH THE ACTIVE EFFLUX PHENOTYPE IN MULTIDRUG-RESISTANT ISOLATES OF HELICOBACTER PYLORI. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2016;9(4):0-0. Available from: https://sid.ir/paper/317604/en

IEEE: Copy

TAHEREH FALSAFI, AZADEH EHSANI, BAHAREH ATTARAN, and VAHID NIKNAM, “ASSOCIATION OF HP1181 AND HP1184 GENES WITH THE ACTIVE EFFLUX PHENOTYPE IN MULTIDRUG-RESISTANT ISOLATES OF HELICOBACTER PYLORI,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 9, no. 4, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/317604/en

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