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Information Journal Paper

Title

Cytotoxic effects of auraptene against a human malignant glioblastoma cell line

Pages

  334-346

Abstract

 Objective: Glioblastoma multiforme (GBM) is the deadliest type of primary Brain tumors, and the survival of patients is estimated to be only about one year. This study, for the first time, investigated the cytotoxic effects of Auraptene on U87 GBM cell line. Materials and Methods: The cellular toxicity was measured by the MTT assay following 24 and 48-hr treatment with different concentrations of Auraptene (0-400μ g/ml). Apoptosis was evaluated by sub-G1 peak in cell cycle analysis of propidium-iodide-stained nuclei. Moreover, to determine the Bax, Bcl-2, MCP-1, NF-κ B, IL1β , and p53 genes expression, we used real-time polymerase chain reaction (RT-PCR). Results: The results revealed that Auraptene reduced the viability of U87 cells concentration-and time-dependently with IC50 values of 108. 9 and 79. 17μ g/ml obtained for 24 and 48-hr treatments, respectively. Also, sub-G1 population was significantly increased following 24 (p<0. 05 and p<0. 001) and 48 (p<0. 001) hours of treatment. The quantitative real-time RT-PCR showed an upregulation in Bax, NF-κ B, IL-1β , and p53 but a down-regulation in MCP-1 and Bcl-2 genes expression. Conclusion: This study showed that Auraptene triggered Apoptosis probably through Bax/Bcl-2 regulation, blocked cell cycle progression and inhibited proliferation in U87 GBM cells. Taken together, Auraptene can be utilized as an effective natural medicine against GBM, after complementary studies.

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    APA: Copy

    Afshari, Amir R., KARIMI ROSHAN, MOSTAFA, SOUKHTANLOO, MOHAMMAD, GHORBANI, AHMAD, RAHMANI, FARZAD, JALILI NIK, MOHAMMAD, Vahedi, Mohammad Mahdi, Hoseini, Azar, Sadeghnia, Hamid R., MOLLAZADEH, HAMID, & MOUSAVI, SEYED HADI. (2019). Cytotoxic effects of auraptene against a human malignant glioblastoma cell line. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), 9(4), 334-346. SID. https://sid.ir/paper/326518/en

    Vancouver: Copy

    Afshari Amir R., KARIMI ROSHAN MOSTAFA, SOUKHTANLOO MOHAMMAD, GHORBANI AHMAD, RAHMANI FARZAD, JALILI NIK MOHAMMAD, Vahedi Mohammad Mahdi, Hoseini Azar, Sadeghnia Hamid R., MOLLAZADEH HAMID, MOUSAVI SEYED HADI. Cytotoxic effects of auraptene against a human malignant glioblastoma cell line. AVICENNA JOURNAL OF PHYTOMEDICINE (AJP)[Internet]. 2019;9(4):334-346. Available from: https://sid.ir/paper/326518/en

    IEEE: Copy

    Amir R. Afshari, MOSTAFA KARIMI ROSHAN, MOHAMMAD SOUKHTANLOO, AHMAD GHORBANI, FARZAD RAHMANI, MOHAMMAD JALILI NIK, Mohammad Mahdi Vahedi, Azar Hoseini, Hamid R. Sadeghnia, HAMID MOLLAZADEH, and SEYED HADI MOUSAVI, “Cytotoxic effects of auraptene against a human malignant glioblastoma cell line,” AVICENNA JOURNAL OF PHYTOMEDICINE (AJP), vol. 9, no. 4, pp. 334–346, 2019, [Online]. Available: https://sid.ir/paper/326518/en

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