THE ROLE OF DEHYDROEPIANDROSTERONE ON LEISHMANIA MAJOR REPLICATION IN BALB/C PERITONEAL MACROPHAGES

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ZAMANI TAGHIZADEH RABE SH.; ZAVARAN HOSSEINI A.; MESBAH NAMIN S.A.R.

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Title

THE ROLE OF DEHYDROEPIANDROSTERONE ON LEISHMANIA MAJOR REPLICATION IN BALB/C PERITONEAL MACROPHAGES

Author(s)

ZAMANI TAGHIZADEH RABE SH. | ZAVARAN HOSSEINI A. | MESBAH NAMIN S.A.R. | Issue Writer Certificate

Keywords

LEISHMANIA MAJORQ2

GLUCOSE-6-PHOSPHATE DEHYDROGENASEQ3

NITRIC OXIDEQ3

BALB/C PERITONEAL MACROPHAGESQ3

DEHYDROEPIANDROSTERONEQ3

Abstract

Introduction: In this study, the relationship between GLUCOSE-6-PHOSPHATE DEHYDROGENASE inhibition in macrophages treated with DEHYDROEPIANDROSTERONE and the amount of NITRIC OXIDE (NO) production and LEISHMANIA MAJOR amastigotes replication in infected macrophages were investigated.Materials and Methods: Peritoneal macrophages of BALB/c mice were isolated and treated with different concentrations (50, 100, 150,200 mm) of DEHYDROEPIANDROSTERONE (DHEA). After incubation for 24 hours, the viability of treated macrophages was measured by MTT assay at 570 nm. G6PD activity was measured in the cell extracts after 24 hours of incubation. Also, macrophages were infected with leishmanial promastigotes and after 18 hours, NO production was determined using Griess-reagent. 100 mm concentration of DHEA was used to study the inhibition of macrophage leishmanicidal activity and the increase in the number of leishmanial amastigotes in these cells from day 1 to 7.Results: Results showed that different concentrations of DHEA significantly increased cell cytotoxicity and decreased G6PD activity and NO production in macrophages. Also, the number of amastigotes in macrophages increased significantly (P£0.001).Conclusion: In this study DEHYDROEPIANDROSTERONE decreased G6PD activity which correlated with the decrease in production of NO and an increase in the cytotoxicity of BALB/c mice peritoneal macrophages. Accordingly, any decrease in G6PD activity lowers NO production and increases cell death. NO has a critical role in leishmanicidal activity of macrophages, so inhibition of G6PD activity decreased leishmanicidat activity of mouse peritoneal macrophages.

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APA: Copy

ZAMANI TAGHIZADEH RABE, SH., ZAVARAN HOSSEINI, A., & MESBAH NAMIN, S.A.R.. (2007). THE ROLE OF DEHYDROEPIANDROSTERONE ON LEISHMANIA MAJOR REPLICATION IN BALB/C PERITONEAL MACROPHAGES. KOWSAR MEDICAL JOURNAL, 12(3), 219-228. SID. https://sid.ir/paper/32699/en

Vancouver: Copy

ZAMANI TAGHIZADEH RABE SH., ZAVARAN HOSSEINI A., MESBAH NAMIN S.A.R.. THE ROLE OF DEHYDROEPIANDROSTERONE ON LEISHMANIA MAJOR REPLICATION IN BALB/C PERITONEAL MACROPHAGES. KOWSAR MEDICAL JOURNAL[Internet]. 2007;12(3):219-228. Available from: https://sid.ir/paper/32699/en

IEEE: Copy

SH. ZAMANI TAGHIZADEH RABE, A. ZAVARAN HOSSEINI, and S.A.R. MESBAH NAMIN, “THE ROLE OF DEHYDROEPIANDROSTERONE ON LEISHMANIA MAJOR REPLICATION IN BALB/C PERITONEAL MACROPHAGES,” KOWSAR MEDICAL JOURNAL, vol. 12, no. 3, pp. 219–228, 2007, [Online]. Available: https://sid.ir/paper/32699/en

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