THE EFFECT OF GARLIC EXTRACT ON NITRIC OXIDE PRODUCTION BY PERITONEAL MACROPHAGES IN MICE

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AHMADI KAZEM; PANDOUNEH A.; ESFAHANI A.A.

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Journal: Iranian Journal of Basic Medical Sciences Year:2000 | Volume:3 | Issue:2 (7) Page(s): 55-60

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Title

THE EFFECT OF GARLIC EXTRACT ON NITRIC OXIDE PRODUCTION BY PERITONEAL MACROPHAGES IN MICE

Author(s)

AHMADI KAZEM | PANDOUNEH A. | ESFAHANI A.A. | Issue Writer Certificate

Keywords

GARLIC EXTRACTQ2

LISHMANIA MAJORQ2

MACROPHAGEQ2

NITRIC OXIDEQ2

Abstract

Previous reports have shown the antibacterial and tumor effects of garlic. In this study, the effect of chloroformic and aqueous extract of garlic was investigated in term of MACROPHAGE NITRIC OXIDE release. Peritoneal MACROPHAGEs were prepared in 1 × 106 cells/ml. Cells were plated out at 1 ×106 cells/well in 24 wells plate in 1 ml RPM I 1640. Cells were incubated at 5% CO2 in air at 37°C for 24 hours in the presence or absence of different concentration of GARLIC EXTRACT (10, 20, 40, 80, 160, 320 µl) as test or control group respectively. In a separate experiment, in addition to different concentration of GARLIC EXTRACT 10000 of LISHMANIA MAJOR (L. major) was added to well. Nitrite was assayed as an indicator of NITRIC OXIDE (NO) by greiss method. The results showed significant differences among groups indicating that GARLIC EXTRACT has the ability to stimulate the MACROPHAGE NITRIC OXIDE release. The results also showed that L. major in vitro has the ability to enhance the NO release by peritoneal MACROPHAGEs in response to GARLIC EXTRACT.

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APA: Copy

AHMADI, KAZEM, PANDOUNEH, A., & ESFAHANI, A.A.. (2000). THE EFFECT OF GARLIC EXTRACT ON NITRIC OXIDE PRODUCTION BY PERITONEAL MACROPHAGES IN MICE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, 3(2 (7)), 55-60. SID. https://sid.ir/paper/52521/en

Vancouver: Copy

AHMADI KAZEM, PANDOUNEH A., ESFAHANI A.A.. THE EFFECT OF GARLIC EXTRACT ON NITRIC OXIDE PRODUCTION BY PERITONEAL MACROPHAGES IN MICE. IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES[Internet]. 2000;3(2 (7)):55-60. Available from: https://sid.ir/paper/52521/en

IEEE: Copy

KAZEM AHMADI, A. PANDOUNEH, and A.A. ESFAHANI, “THE EFFECT OF GARLIC EXTRACT ON NITRIC OXIDE PRODUCTION BY PERITONEAL MACROPHAGES IN MICE,” IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES, vol. 3, no. 2 (7), pp. 55–60, 2000, [Online]. Available: https://sid.ir/paper/52521/en

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