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Information Journal Paper

Title

POLYMERASE CHAIN REACTION METHOD FOR THE RAPID DETECTION OF VIRULENT SHIGELLA SPP.

Pages

  134-137

Keywords

PCR 

Abstract

 Bacillary dysentery, or shigellosis, is a disease of humans in which the colonic epithelium is invaded by bacteria and subjected to inflammatory destruction. The aim of this study was to develop a polymerase chain reaction (PCR) test for detection of virulent SHIGELLA SPP. For this purpose, the primers were designed to amplify a 526-bp internal region of the SHIGELLA SPP. ICSA gene, which encodes ICSA (intracellular spread)/VirG protein, a 116-kDa surface exposed outer membrane protein that mediates actin polymerization to aid bacterial movement inside the cell. The use of PCR to amplify a specific ICSA gene fragment serves as a highly specific and sensitive method to detect virulent bacteria of the genus Shigella. Specific DNA band was obtained by using isolated plasmid DNA of Shigella and a bacterial suspension. Amplification of extracted DNA from all other genera of the family Enterobacteriaceae and various other gram-positive bacteria yielded negative results. Therefore this PCR method, can serve as a routine protocol for detecting and identifying virulent SHIGELLA SPP. from clinical samples.

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    Cite

    APA: Copy

    ALIPOUR, MAJID, TALEBJANNAT, MARYAM, & NABIUNI, MOHAMMAD. (2012). POLYMERASE CHAIN REACTION METHOD FOR THE RAPID DETECTION OF VIRULENT SHIGELLA SPP.. INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY, 2(1), 134-137. SID. https://sid.ir/paper/328054/en

    Vancouver: Copy

    ALIPOUR MAJID, TALEBJANNAT MARYAM, NABIUNI MOHAMMAD. POLYMERASE CHAIN REACTION METHOD FOR THE RAPID DETECTION OF VIRULENT SHIGELLA SPP.. INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY[Internet]. 2012;2(1):134-137. Available from: https://sid.ir/paper/328054/en

    IEEE: Copy

    MAJID ALIPOUR, MARYAM TALEBJANNAT, and MOHAMMAD NABIUNI, “POLYMERASE CHAIN REACTION METHOD FOR THE RAPID DETECTION OF VIRULENT SHIGELLA SPP.,” INTERNATIONAL JOURNAL OF MOLECULAR AND CLINICAL MICROBIOLOGY, vol. 2, no. 1, pp. 134–137, 2012, [Online]. Available: https://sid.ir/paper/328054/en

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