THE EFFECT OF 20% HEATED AND NON-HEATED FOLLICULAR FLUID ON MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES IN VITRO

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IMANI H.; REZAZADEH M.

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Journal: KOWSAR MEDICAL JOURNAL Year:2004 | Volume:9 | Issue:Part 2 Page(s): 119-126

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Title

THE EFFECT OF 20% HEATED AND NON-HEATED FOLLICULAR FLUID ON MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES IN VITRO

Author(s)

IMANI H. | REZAZADEH M. | Issue Writer Certificate

Keywords

IN VITRO MATURATIONQ3

FOLLICULAR FLUIDQ3

TRANSFERRINQ4

INSULINQ3

IMMATURE OOCYTESQ4

MOUSEQ4

Abstract

Objective. In the mice, rate of fertilization and development are important in oocyte matured and a closer imitation of in-vivo conditions during oocyte maturation might be beneficial. The aims of the present study were to investigate whether lmmature mouse oocytes can be matured in-vitro in 20% heated and non-heated human FOLLICULAR FLUID and TRANSFERRIN and INSULIN affected FOLLICULAR FLUID on oocyte maturation and development. Materials and Methods. IMMATURE OOCYTES were isolated mechanically from 4-6 weeks old NMRI mice ovaries. Oocytes washed and transferred to the drops of medium in following experiment groups for 24 hours: Group I: 207 IMMATURE OOCYTES were cultured in DMEM-Hams F12 containing 20% heated human FOLLICULAR FLUID (hHFF). Group II: 189 IMMATURE OOCYTES were cultured in DMEM-Hams F12 containing 20% Non-heated Human FOLLICULAR FLUID (NhHFF). Group III: 242 IMMATURE OOCYTES were cultured in DMEM-Hams F12 containing 20% hHFF, 5mg/ml INSULIN and 10mg/ml TRANSFERRIN. Group IV: 175 IMMATURE OOCYTES cultured in DMEM-Hams F12 containing 20% NhHFF. 5mg/ml lnsulin and 10mg/ml TRANSFERRIN. Results. The overall maturation was significantly higher for oocytes in group III (610/5%) that other groups. The maturation rates were: 610/5% (group III), 41% (group I), 27/4% (group IV) and 10/6% (group II) respectively. The 2-cell formation embryo rates were 56% (group III), 16/7% (group IV). 15/5% (group I) and 5% (group II). Conclusion. The results demonstrate that (1) mouse IMMATURE OOCYTES can be matured during culture in above medium containing 20% hHFF, NhHFF, INSULIN and TRANSFERRIN (2), maturating and development was higher in presente of 20% hHFF, TRANSFERRIN and Insulin, (3) the higher regulation of meiotic resumption during in-vitro culture of mouse oocytes might be related to the presence of INSULIN and TRANSFERRIN.

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APA: Copy

IMANI, H., & REZAZADEH, M.. (2004). THE EFFECT OF 20% HEATED AND NON-HEATED FOLLICULAR FLUID ON MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES IN VITRO. KOWSAR MEDICAL JOURNAL, 9(Part 2), 119-126. SID. https://sid.ir/paper/32877/en

Vancouver: Copy

IMANI H., REZAZADEH M.. THE EFFECT OF 20% HEATED AND NON-HEATED FOLLICULAR FLUID ON MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES IN VITRO. KOWSAR MEDICAL JOURNAL[Internet]. 2004;9(Part 2):119-126. Available from: https://sid.ir/paper/32877/en

IEEE: Copy

H. IMANI, and M. REZAZADEH, “THE EFFECT OF 20% HEATED AND NON-HEATED FOLLICULAR FLUID ON MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES IN VITRO,” KOWSAR MEDICAL JOURNAL, vol. 9, no. Part 2, pp. 119–126, 2004, [Online]. Available: https://sid.ir/paper/32877/en

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