IN VITRO DEVELOPMENT AND FERTILIZATION OF VITRIFIED EARLY NEONATE PREANTRAL MOUSE FOLLICLES

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IMANI H.; REZAZADEH VALOUJERDI M.; HISSEINI A.; ASHTIANI S.K.

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Paper Information

Journal: KOWSAR MEDICAL JOURNAL Year:2002 | Volume:7 | Issue:part 1 Page(s): 41-52

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Title

IN VITRO DEVELOPMENT AND FERTILIZATION OF VITRIFIED EARLY NEONATE PREANTRAL MOUSE FOLLICLES

Author(s)

IMANI H. | REZAZADEH VALOUJERDI M. | HISSEINI A. | ASHTIANI S.K. | Issue Writer Certificate

Keywords

PREANTRAL FOLLICLEQ4

MOUSEQ3

VITRIFIEDQ3

DEVELOPMENTQ3

FERTILIZATIONQ4

Abstract

In this research the potential DEVELOPMENT of VITRIFIED early preantral of neonate MOUSE follicle was studied. Early PREANTRAL FOLLICLEs with the diameter of 100-130 mm were isolated mechanically from 14 days old NMRY mice ovaries.Follicles were VITRIFIED using PBI containing 30% WN Ficoll, 0.5 M sucrose, 105 mg BSA, 10.7 WN Acetamide and 40% V/V ethylene Glycol (EGFA40) as a cryopreservation solution and then plunged into liquid nitrogen, Stored for two weeks. Thawing was performed in one step manner using 0.5 M Sucrose in BPI and then washed with PBI, transferred to the drops of DMEM/Hams F12 containing 5% FCS. 253 intact VITRIFIED PREANTRAL FOLLICLE were cultured in drops in 20 ml of culture medium containing 5 mg/ml insulin, 10 mg/ml transferrin and 100 mlU/ml rFSH. two day after culturing, survive follicles evaluated under invert microscope. preantral follicar DEVELOPMENT followed up to day 12 and culture media renewed every two days. At day 12, preovolatory follicles were stimulated with 1.5 IU/ml hCG. After 24 hours, mature, ovulated oocytes inseminated with the capacitated epidydimal sperms as the same strain of mice. Germinal vesicle (GV), germinal vesicle breakdown (GVB), extrusion of first polar body, FERTILIZATION and DEVELOPMENT of two cells embryos in vitrified were evaluated by using inverte microscope. results showed that after 48h, 91.2% of VITRIFIED follicles were survived and at the 6th day of culturing, follicular follicles changes to outgrowth follicle and finally 15.9% of AFF follicles were ovulated and 42.1% of oocytes were showen to be 2-cell embryos. There fore it can be conclued that PREANTRAL FOLLICLE of neonate as a VITRIFIED can develop in DMEM-Hams F12 toward germinal vesicle breakdown successfully but their further progression need to be investigated.

Cites

THE EFFECT OF 20% HEATED AND NON-HEATED FOLLICULAR FLUID ON MATURATION AND DEVELOPMENT OF IMMATURE MOUSE OOCYTES IN VITRO

References

بررسی از سرگیری میوز در تخمک فولیکول اولیه موش و رشد لقاح آنها در آزمایشگاه

Cite

APA: Copy

IMANI, H., REZAZADEH VALOUJERDI, M., HISSEINI, A., & ASHTIANI, S.K.. (2002). IN VITRO DEVELOPMENT AND FERTILIZATION OF VITRIFIED EARLY NEONATE PREANTRAL MOUSE FOLLICLES. KOWSAR MEDICAL JOURNAL, 7(part 1), 41-52. SID. https://sid.ir/paper/33050/en

Vancouver: Copy

IMANI H., REZAZADEH VALOUJERDI M., HISSEINI A., ASHTIANI S.K.. IN VITRO DEVELOPMENT AND FERTILIZATION OF VITRIFIED EARLY NEONATE PREANTRAL MOUSE FOLLICLES. KOWSAR MEDICAL JOURNAL[Internet]. 2002;7(part 1):41-52. Available from: https://sid.ir/paper/33050/en

IEEE: Copy

H. IMANI, M. REZAZADEH VALOUJERDI, A. HISSEINI, and S.K. ASHTIANI, “IN VITRO DEVELOPMENT AND FERTILIZATION OF VITRIFIED EARLY NEONATE PREANTRAL MOUSE FOLLICLES,” KOWSAR MEDICAL JOURNAL, vol. 7, no. part 1, pp. 41–52, 2002, [Online]. Available: https://sid.ir/paper/33050/en

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