EFFECT OF NICOTINIC RECEPTORS AGONIST AND ANTAGONIST ON ACETYLCOLINE STERASE EXPRITION IN SHSY5Y CELLS

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MEHRANI H.A.; SMALL D.

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Journal: KOWSAR MEDICAL JOURNAL Year:2005 | Volume:10 | Issue:2 Page(s): 79-88

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Title

EFFECT OF NICOTINIC RECEPTORS AGONIST AND ANTAGONIST ON ACETYLCOLINE STERASE EXPRITION IN SHSY5Y CELLS

Author(s)

MEHRANI H.A. | SMALL D. | Issue Writer Certificate

Keywords

ACETYLCHOLINEQ3

ACETYLCHOLINESTERASEQ4

Α 7-NICOTINIC RECEPTORQ4

AMYLOID ΒQ4

SHSY5Y AND PC12 CELLSQ4

Abstract

Objective: The aim of the present study was to examine the mechanism by which β-amyloid interact with nicotinic receptors and changes the level of cetylcholinesterase (AChE) in Alzheimer's disease. Materials and Methods: SHSY5Y and PC12 human neuroblastoma and pheochromocytoma cells were cultured and effects of A β, and nicotinic receptor agonists and antagonists were investigated. Results: A β 1-42 significantly increased AChE in SHSY5Y cells. The effect of A β was blocked by nicotine and α-bungarotoxin (α -Bgt) a selective antagonist of α 7 nicotinic ACETYLCHOLINE receptor (nAChR). Nicotine alone had no effect on AChE activity, but ACETYLCHOLINE, choline and carbamoylcholine all increased AChE activity in these cells. Nicotinic receptor antagonists such as α -Bgt, methellycaconitine (MLA), ecamylamine and dihydro β -erythroidin (DH β E) did not have any effect on AChE activity. In contrast, selective α 7 nAChR antagonist's α -Bgt and MLA in concomitant with 10 µM nicotine, significantly increased AChE activity in SHSY5Y cells, but non- α 7 preferring antagonist, DH β E was not effective. This activation was not seen in PC12 cells. ACETYLCHOLINE (but not nicotine) increased α 7 nAChR density in SHSY5Y cell membrane. Conclusion: These results demonstrate that increase in AChE activity in these cells is due to α 7 nAChR.

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APA: Copy

MEHRANI, H.A., & SMALL, D.. (2005). EFFECT OF NICOTINIC RECEPTORS AGONIST AND ANTAGONIST ON ACETYLCOLINE STERASE EXPRITION IN SHSY5Y CELLS. KOWSAR MEDICAL JOURNAL, 10(2), 79-88. SID. https://sid.ir/paper/32957/en

Vancouver: Copy

MEHRANI H.A., SMALL D.. EFFECT OF NICOTINIC RECEPTORS AGONIST AND ANTAGONIST ON ACETYLCOLINE STERASE EXPRITION IN SHSY5Y CELLS. KOWSAR MEDICAL JOURNAL[Internet]. 2005;10(2):79-88. Available from: https://sid.ir/paper/32957/en

IEEE: Copy

H.A. MEHRANI, and D. SMALL, “EFFECT OF NICOTINIC RECEPTORS AGONIST AND ANTAGONIST ON ACETYLCOLINE STERASE EXPRITION IN SHSY5Y CELLS,” KOWSAR MEDICAL JOURNAL, vol. 10, no. 2, pp. 79–88, 2005, [Online]. Available: https://sid.ir/paper/32957/en

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