PATTERNS OF CYTOKINE PRODUCTION IN BALB/C MICE LYMPH NODE CELLS REACTIVATED BY LEISHMANIA MAJOR EXCRETORY AND SECRETORY PRODUCTS

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MAHMOUDZADEH POURNAKI ABAS; MEHRANI H.A.; AHMADI KAZEM; SHIRBAZOU SH.; FATAHI BAFGHI A.

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Journal: KOWSAR MEDICAL JOURNAL Year:2008 | Volume:12 | Issue:4 Page(s): 315-324

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Title

PATTERNS OF CYTOKINE PRODUCTION IN BALB/C MICE LYMPH NODE CELLS REACTIVATED BY LEISHMANIA MAJOR EXCRETORY AND SECRETORY PRODUCTS

Author(s)

Keywords

LEISHMANIA MAJORQ3

EXCRETOR- SECRETORY PRODUCTSQ3

CYTOKINESQ3

Abstract

After recovery from cutaneous leishmaniasis protective immunity occurs in most situations. So, vaccination with excretory and secretory antigens (ES-Ag) could leads to protective immunity. In this study the effects of excreted- secreted products of LEISHMANIA MAJOR on cytokine production pattern in BALB/c lymph node cells were evaluated. five groups (n=12 in each group) of BALB/c mice were randomly assigned to WA(whole antigen), 24hr ES-Ag, 0hr ES-Ag, adjuvant alone and control groups, and challenged with LEISHMANIA MAJOR promastigotes. In the second months of wound appearance, the animals were decapitated and drainage lymph nods cells were removed and cultured for CYTOKINES assay. After 48 hr of incubation with 24hr ES-Ag, the cells culture supernatants were removed and assayed for CYTOKINES levels. The IL-4 and IL-10 CYTOKINES levels in WA animals group were significantly higher than the other groups. In animals receiving 24hr ES-Ag the IL-2 level was significantly high comparing to the other experimental groups.

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APA: Copy

MAHMOUDZADEH POURNAKI, ABAS, MEHRANI, H.A., AHMADI, KAZEM, SHIRBAZOU, SH., & FATAHI BAFGHI, A.. (2008). PATTERNS OF CYTOKINE PRODUCTION IN BALB/C MICE LYMPH NODE CELLS REACTIVATED BY LEISHMANIA MAJOR EXCRETORY AND SECRETORY PRODUCTS. KOWSAR MEDICAL JOURNAL, 12(4), 315-324. SID. https://sid.ir/paper/33020/en

Vancouver: Copy

MAHMOUDZADEH POURNAKI ABAS, MEHRANI H.A., AHMADI KAZEM, SHIRBAZOU SH., FATAHI BAFGHI A.. PATTERNS OF CYTOKINE PRODUCTION IN BALB/C MICE LYMPH NODE CELLS REACTIVATED BY LEISHMANIA MAJOR EXCRETORY AND SECRETORY PRODUCTS. KOWSAR MEDICAL JOURNAL[Internet]. 2008;12(4):315-324. Available from: https://sid.ir/paper/33020/en

IEEE: Copy

ABAS MAHMOUDZADEH POURNAKI, H.A. MEHRANI, KAZEM AHMADI, SH. SHIRBAZOU, and A. FATAHI BAFGHI, “PATTERNS OF CYTOKINE PRODUCTION IN BALB/C MICE LYMPH NODE CELLS REACTIVATED BY LEISHMANIA MAJOR EXCRETORY AND SECRETORY PRODUCTS,” KOWSAR MEDICAL JOURNAL, vol. 12, no. 4, pp. 315–324, 2008, [Online]. Available: https://sid.ir/paper/33020/en

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