EFFECT OFATNRT2.1 TRANSGENE ON HATS NITRATE UPTAKE IN TRANSGENICNICOTIANA PLUMBAGINIFOLIA

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ZOUFAN PARZHAK; SHARIATI MANSOUR

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Journal: PROGRESS IN BIOLOGICAL SCIENCES Year:2011 | Volume:1 | Issue:1 Page(s): 1-9

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Title

EFFECT OFATNRT2.1 TRANSGENE ON HATS NITRATE UPTAKE IN TRANSGENICNICOTIANA PLUMBAGINIFOLIA

Author(s)

ZOUFAN PARZHAK | SHARIATI MANSOUR | Issue Writer Certificate

Keywords

GLUTAMINE

IHATS

NITRATE TRANSPORTER

TOBACCO

Abstract

To investigate the impact of overexpression ofAtNRT2.1 transgene from Arabidopsis on nitrate uptake rate and to understand the regulation of endogenous HATS by nitrate and glutamine amino acid (Gln) in tobacco plants, wild-type and transgenic (F line) plants grown on soil for 4 weeks were transferred to hydroponic culture in a controlled-environment with a 16/8h L: D photoperiod at 24oC/20oC, 70% relative humidity and 150 mmol. m-2. s-1 light intensity. Nitrate uptake over time was studied by the ion depletion method. Nitrate uptake in the 2 h exposure to 150 µM nitrate showed an increase in the transgenic line compared to the wild-type plant. Subsequently the uptake trend followed a similar pattern in wild-type plant and transgenic plants. Pretreatment with high concentrations of nitrate (10 mM) increased rate of uptake in both wild-type and transgenic plants.No marked differences between the two genotypes were observed with respect to HATS activity.Glutamine decreased the rate of nitrate uptake in both wild-type and, to a lesser extent, in transgenic plants.

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APA: Copy

ZOUFAN, PARZHAK, & SHARIATI, MANSOUR. (2011). EFFECT OFATNRT2.1 TRANSGENE ON HATS NITRATE UPTAKE IN TRANSGENICNICOTIANA PLUMBAGINIFOLIA. PROGRESS IN BIOLOGICAL SCIENCES, 1(1), 1-9. SID. https://sid.ir/paper/331175/en

Vancouver: Copy

ZOUFAN PARZHAK, SHARIATI MANSOUR. EFFECT OFATNRT2.1 TRANSGENE ON HATS NITRATE UPTAKE IN TRANSGENICNICOTIANA PLUMBAGINIFOLIA. PROGRESS IN BIOLOGICAL SCIENCES[Internet]. 2011;1(1):1-9. Available from: https://sid.ir/paper/331175/en

IEEE: Copy

PARZHAK ZOUFAN, and MANSOUR SHARIATI, “EFFECT OFATNRT2.1 TRANSGENE ON HATS NITRATE UPTAKE IN TRANSGENICNICOTIANA PLUMBAGINIFOLIA,” PROGRESS IN BIOLOGICAL SCIENCES, vol. 1, no. 1, pp. 1–9, 2011, [Online]. Available: https://sid.ir/paper/331175/en

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