RAPID DETECTION OF MUTATIONS IN CODON 43 OF RPSL GENE BY RFLP METHOD WITH BSAJI AND MBOOII RESTRICTION ENZYMES ASSOCIATED WITH RESISTANCE TO STREPTOMYCIN IN CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS

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ARJOMANDZADEGAN M.; GERAVAND S.; AHMADI A.; SADRNIA M.; KAHBAZI M.

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Journal Paper

Paper Information

Journal: IRANIAN SOUTH MEDICAL JOURNAL (ISMJ) Year:2016 | Volume:18 | Issue:6 Page(s): 1115-1123

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Title

RAPID DETECTION OF MUTATIONS IN CODON 43 OF RPSL GENE BY RFLP METHOD WITH BSAJI AND MBOOII RESTRICTION ENZYMES ASSOCIATED WITH RESISTANCE TO STREPTOMYCIN IN CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS

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Keywords

DRUG RESISTANCEQ2

STREPTOMYCINQ2

PCR-RFLPQ2

BSAJIQ2

Abstract

Abstract Background: STREPTOMYCIN is one of the most efficient treatments of tuberculosis taht increasingly reported its DRUG RESISTANCE. The most common mutations associated with DRUG RESISTANCE to STREPTOMYCIN of Mycobacterium Tuberculosis, causes tuberculosis, and occurs at codons 43 and 88 fo rpsL gene. The purpose of this study is study of alterations in rpsL gene with two restriction enzymes BSAJI and MbooII related to drug resistant to STREPTOMYCIN in clinical isolates of mycobacterium tuberculosis.Materials and Methods: This study was performed using 71 clinical isolates of Mycobacterium tuberculosis. Molecular PCR-RFLP methods were designed with two enzymes BSAJI and MbooII for mutation analysis at codon 43 of rpsL, in resistant and susceptible strains, respectively. Finally, the results were compared with sequencing and phenotype strains.Results: 25 subjects were studied with enzyme BSAJI. This enzyme is capable of detection 64 percent of resistant and all sensitive strains. 46 strains were examined by enzyme MboII. This enzyme detected almost 91 percent of sensitive strains. MboII is selected for detection of sensitive strains (unlike BSAJI). Results of sequencing rpsL genes in investigated strains, showed fully consistent with the results of PCR-RFLP and proved mutation in codon of 43 of thin genes in studied strains.Conclusion: The results showed that the PCR-RFLP with designed restriction enzymes can be used as a rapid, simple assay, and has high sensitivity for differentiation of Mycobacterium tuberculosis strains that are resistance to STREPTOMYCIN.

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References

PREVALENCE OF PULMONARY TUBERCULOSIS AMONG PATIENTS WITH ANTHRACOFIBROSIS IN ZAHEDAN

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APA: Copy

ARJOMANDZADEGAN, M., GERAVAND, S., AHMADI, A., SADRNIA, M., & KAHBAZI, M.. (2016). RAPID DETECTION OF MUTATIONS IN CODON 43 OF RPSL GENE BY RFLP METHOD WITH BSAJI AND MBOOII RESTRICTION ENZYMES ASSOCIATED WITH RESISTANCE TO STREPTOMYCIN IN CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), 18(6), 1115-1123. SID. https://sid.ir/paper/34084/en

Vancouver: Copy

ARJOMANDZADEGAN M., GERAVAND S., AHMADI A., SADRNIA M., KAHBAZI M.. RAPID DETECTION OF MUTATIONS IN CODON 43 OF RPSL GENE BY RFLP METHOD WITH BSAJI AND MBOOII RESTRICTION ENZYMES ASSOCIATED WITH RESISTANCE TO STREPTOMYCIN IN CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS. IRANIAN SOUTH MEDICAL JOURNAL (ISMJ)[Internet]. 2016;18(6):1115-1123. Available from: https://sid.ir/paper/34084/en

IEEE: Copy

M. ARJOMANDZADEGAN, S. GERAVAND, A. AHMADI, M. SADRNIA, and M. KAHBAZI, “RAPID DETECTION OF MUTATIONS IN CODON 43 OF RPSL GENE BY RFLP METHOD WITH BSAJI AND MBOOII RESTRICTION ENZYMES ASSOCIATED WITH RESISTANCE TO STREPTOMYCIN IN CLINICAL ISOLATES OF MYCOBACTERIUM TUBERCULOSIS,” IRANIAN SOUTH MEDICAL JOURNAL (ISMJ), vol. 18, no. 6, pp. 1115–1123, 2016, [Online]. Available: https://sid.ir/paper/34084/en

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