MOLECULAR DETECTION OF SHIGELLA SPP. CONTAMINATION IN READY-TO-EAT SALAD SAMPLES IN WEST OF TEHRAN

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TAHMASEBI TEHRANI SALOUMEH; HARZANDI NASER; Jabalameli Leila

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Journal: International Journal of Enteric Pathogens Year:2018 | Volume:6 | Issue:2 Page(s): 41-44

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Information Journal Paper

Title

MOLECULAR DETECTION OF SHIGELLA SPP. CONTAMINATION IN READY-TO-EAT SALAD SAMPLES IN WEST OF TEHRAN

Author(s)

TAHMASEBI TEHRANI SALOUMEH | HARZANDI NASER | Jabalameli Leila | Issue Writer Certificate

Keywords

SHIGELLA

PRE-PACKED SALADS

IPAH GENE

TEHRAN

PCR

Abstract

Background: SHIGELLA bacteria can infect human body by taking contaminated food and water, and are transmitted from person to person. Human body is the only natural host for these bacteria.Objective: The aim of this study was to detect SHIGELLA contamination in pre-packed samples of salads at restaurants in western regions of TEHRAN, Iran, using polymerase chain reaction (PCR) method.Materials and Methods: To conduct this research, 90 samples were purchased from the restaurants during the period of June to November 2016. The samples were cut into very small pieces, homogenized and a 25 g portion of these samples was added to 225 mL of SHIGELLA broth media containing novobiocin and incubated for 24 hours. Then DNA of cultured samples was extracted using DNPTM kit (CinnaGen, Iran). PCR method was optimized for amplification of 613 bp segment of IPAH GENE and performed on extracted DNA of all samples (before and after enrichment in SHIGELLA broth).Results: SHIGELLA contamination was detected in 7 (7.8%) and 20 (22.2%) of the tested samples before and after the enrichment, respectively.Conclusion: The results showed the contamination with SHIGELLA bacteria in remarkable percentage of the samples and revealed the necessity of more attention and supervision in the processes of production and distribution of PRE-PACKED SALADS.

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APA: Copy

TAHMASEBI TEHRANI, SALOUMEH, HARZANDI, NASER, & Jabalameli, Leila. (2018). MOLECULAR DETECTION OF SHIGELLA SPP. CONTAMINATION IN READY-TO-EAT SALAD SAMPLES IN WEST OF TEHRAN. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, 6(2), 41-44. SID. https://sid.ir/paper/347607/en

Vancouver: Copy

TAHMASEBI TEHRANI SALOUMEH, HARZANDI NASER, Jabalameli Leila. MOLECULAR DETECTION OF SHIGELLA SPP. CONTAMINATION IN READY-TO-EAT SALAD SAMPLES IN WEST OF TEHRAN. INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN[Internet]. 2018;6(2):41-44. Available from: https://sid.ir/paper/347607/en

IEEE: Copy

SALOUMEH TAHMASEBI TEHRANI, NASER HARZANDI, and Leila Jabalameli, “MOLECULAR DETECTION OF SHIGELLA SPP. CONTAMINATION IN READY-TO-EAT SALAD SAMPLES IN WEST OF TEHRAN,” INTERNATIONAL JOURNAL OF ENTERIC PATHOGEN, vol. 6, no. 2, pp. 41–44, 2018, [Online]. Available: https://sid.ir/paper/347607/en

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