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Information Journal Paper

Title

NEW AND EFFICIENT METHOD FOR DNA EXTRACTION FROM HUMAN SKELETAL REMAINS USABLE IN DNA TYPING

Pages

  609-614

Abstract

 In historical cases, mass disasters, missing person’s IDENTIFICATION and ancient DNAinvestigations, BONE and teeth samples are often the best and the only biologicalmaterial available for DNA typing. This is because of the physical and chemicalnature of the protein-mineral matrix of BONE which is relatively resistant to theadverse environmental effects and biological attacks. Most BONE extraction protocolsused in the forensic laboratories involve an incubation period of BONE powderin extraction buffer for proteinase digestion, followed by the collection of the supernatant,and the elimination of large quantities of undigested BONE powder. Herewe demonstrate an extremely effective protocol for recovery of DNA. This is performedin a method that retains and concentrates all the reagent volume for completeDNA recovery. For our study, we selected challenging BONE samples of skeletonremains of the martyred individuals in Iraq’s imposed war on I.R. Iran (1980-1988). The BONEs that were extracted with our new protocol showed that this newprotocol significantly enhances the quantity of DNA that can be used for amplificationfrom degraded skeletal remains. At the same time we tested in parallel thesamples by using of QIAamp DNA Investigator Kit and attained the best results byusing new protocol. In fact, our new DNA EXTRACTION method is based on previousstandard methods such as Chelex and salting out. We have used this technique tosuccessfully recover authentic DNA Typing from extremely challenging samplesthat failed repeatedly using the standard protocols. However, the amount of recoveredDNA was very small but it was possible to extract genomic DNA from thesechallenging BONE samples. The results indicated that our procedure for DNA EXTRACTIONalthough yielded little amount of genomic DNA; however, it was pure DNAthat can be used for further analysis such as PCR amplification and DNA profiling.Since the new procedure is fast and needed less time than previously standard procedures,we have named it FDEB (Fast DNA EXTRACTION of BONE).

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  • Cite

    APA: Copy

    MOHAMMADI, ALI, GHORBANI ALVANEGH, AKBAR, KHAFAEI, MOSTAFA, HABIBI AZARIAN, SAJJAD, NADERI, MAHDI, KIYANI, EBRAHIM, MIRI, ALI, BAHMANI, HASAN, RAMEZANI, MARYAM, & TAVALLAEI, MAHMOOD. (2017). NEW AND EFFICIENT METHOD FOR DNA EXTRACTION FROM HUMAN SKELETAL REMAINS USABLE IN DNA TYPING. JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS, 4(2), 609-614. SID. https://sid.ir/paper/352956/en

    Vancouver: Copy

    MOHAMMADI ALI, GHORBANI ALVANEGH AKBAR, KHAFAEI MOSTAFA, HABIBI AZARIAN SAJJAD, NADERI MAHDI, KIYANI EBRAHIM, MIRI ALI, BAHMANI HASAN, RAMEZANI MARYAM, TAVALLAEI MAHMOOD. NEW AND EFFICIENT METHOD FOR DNA EXTRACTION FROM HUMAN SKELETAL REMAINS USABLE IN DNA TYPING. JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS[Internet]. 2017;4(2):609-614. Available from: https://sid.ir/paper/352956/en

    IEEE: Copy

    ALI MOHAMMADI, AKBAR GHORBANI ALVANEGH, MOSTAFA KHAFAEI, SAJJAD HABIBI AZARIAN, MAHDI NADERI, EBRAHIM KIYANI, ALI MIRI, HASAN BAHMANI, MARYAM RAMEZANI, and MAHMOOD TAVALLAEI, “NEW AND EFFICIENT METHOD FOR DNA EXTRACTION FROM HUMAN SKELETAL REMAINS USABLE IN DNA TYPING,” JOURNAL OF APPLIED BIOTECHNOLOGY REPORTS, vol. 4, no. 2, pp. 609–614, 2017, [Online]. Available: https://sid.ir/paper/352956/en

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