GROWTH INHIBITION OF SECRETED DNA OF HUMAN MYELOID CELL LINE (HL-60) ON PERIPHERAL BLOOD LYMPHOCYTE

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ABOU ALHASANI A.; CHIAO J.W.

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Paper Information

Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2004 | Volume:14 | Issue:43 Page(s): 9-16

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Title

GROWTH INHIBITION OF SECRETED DNA OF HUMAN MYELOID CELL LINE (HL-60) ON PERIPHERAL BLOOD LYMPHOCYTE

Author(s)

ABOU ALHASANI A. | CHIAO J.W. | Issue Writer Certificate

Keywords

DNAQ4

HL-60 CELLSQ4

LYPHOCYTESQ4

LEUKEMIA-MYELOCYTIC-ACTE-PREVENTION CONTROLQ4

IMMUNOSU PPRESSIVE AGENTSQ4

Abstract

Background and purpose: So far many protein factors capable of inhibiting the immune system are secreted by different cancer cell lines in the culture supernatant and characterized. In this report, the release of DNA fragments into the culture supernatant of human promyelocytic cell line, HL-60, with immunosuppressive activity was investigated.Materials and methods: HL-60 culture supernatant was concentrated and applied to ion-exchange DEAE-Sepharose CL-6B column. The column was eluted with NaCl gradient and the fractions were collected for assay. Fractions with immunosuppressive activity were purified with HPLC.Result: The data show that trypsin and RNase have no effect on antiproliferation, but DNAse destroyed the immunosuppressive activity. The DNA with irreversible immuno-suppressive activity was found to be double-stranded with the molecular weight range between 0.6 and 1.5 kilobases. This DNA did not change the cell number and viability and as a result was not cytotoxic. Cell cycle analysis has suggested that DNA molecule could arrest the mitogen-stimulated lymphocytes in G1 phase, and prevent the cells from entering into the proliferating S phase.Conclusion: These data show that DNA like immunosuppressive proteins could inhibit the proliferation of lymphocytes as well as the proliferation of some cancer cells.

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APA: Copy

ABOU ALHASANI, A., & CHIAO, J.W.. (2004). GROWTH INHIBITION OF SECRETED DNA OF HUMAN MYELOID CELL LINE (HL-60) ON PERIPHERAL BLOOD LYMPHOCYTE. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 14(43), 9-16. SID. https://sid.ir/paper/357707/en

Vancouver: Copy

ABOU ALHASANI A., CHIAO J.W.. GROWTH INHIBITION OF SECRETED DNA OF HUMAN MYELOID CELL LINE (HL-60) ON PERIPHERAL BLOOD LYMPHOCYTE. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2004;14(43):9-16. Available from: https://sid.ir/paper/357707/en

IEEE: Copy

A. ABOU ALHASANI, and J.W. CHIAO, “GROWTH INHIBITION OF SECRETED DNA OF HUMAN MYELOID CELL LINE (HL-60) ON PERIPHERAL BLOOD LYMPHOCYTE,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 14, no. 43, pp. 9–16, 2004, [Online]. Available: https://sid.ir/paper/357707/en

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