IN VITRO BIOCOMPATIBILITY OF THREE ORTHODONTIC COMPOSITE RESINS ON HUMAN ORAL
FIBROBLASTS

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SADEGHIAN SOUSAN; NASRESFAHANI MOHAMMAD HOSSEIN; VATANI M.

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2007 | Volume:15 | Issue:2 Page(s): 31-38

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Title

IN VITRO BIOCOMPATIBILITY OF THREE ORTHODONTIC COMPOSITE RESINS ON HUMAN ORAL FIBROBLASTS

Author(s)

SADEGHIAN SOUSAN | NASRESFAHANI MOHAMMAD HOSSEIN | VATANI M. | Issue Writer Certificate

Keywords

BIOCOMPATIBILITYQ3

HUMAN ORAL FIBROBLASTSQ3

ORTHODONTIC COMPOSITE RESINQ3

MTT ASSAYQ2

Abstract

Introduction: In orthodontic therapy, different materials are used and their presence in oral cavity may modify their properties. The purpose of this study was to evaluate the direct and indirect BIOCOMPATIBILITY of three ORTHODONTIC COMPOSITE RESINs on culture of HUMAN ORAL FIBROBLASTS.Methods: This was an interventional experimental study. The BIOCOMPATIBILITY of three ORTHODONTIC COMPOSITE RESINs including two kinds of self cure composite resins(Unite,3M and Fantastic, Zar Dent)and one kind of fight cure composite resin (Transbond,3M) were studied. After a releasing period of each material in the culture medium for 20 days, the viability of oral cultivated fibroblasts was compared to negative control(Teflon) and together with MTT ASSAY(3-(4,5dimethylthiazolyl-2) -2,5-diphenyltetrazolium bromide). In order to evaluate the direct BIOCOMPATIBILITY, after direct contacting of composite resins with cell culture, the viability of oral cells was evaluated with invert phase contrast microscope.Results: The results of MTT ASSAY showed the BIOCOMPATIBILITY of light cure composite (P.V==0.114)In comparison to control group. There were significant deterrence’s between BIOCOMPATIBILITY of self cure composite resins and control group (P.V=0.029). On the other hand, significant statistical difference was not seen between BIOCOMPATIBILITY of three composites. The results of direct method showed grade 0 for light cure composite, gradel for fantastic and grade 2 for unite (3M).Conclusion: This study suggested that under these experimental conditions, chemically cured(no-mix) composite resins were more cytotoxic than light cured ones in both direct and indirect methods. Further investigations into the int1uenceof the modes of polymerization and biologic effects of primers on cytotoxic effect and animal studies are warranted to verity its clinical implication.

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APA: Copy

SADEGHIAN, SOUSAN, NASRESFAHANI, MOHAMMAD HOSSEIN, & VATANI, M.. (2007). IN VITRO BIOCOMPATIBILITY OF THREE ORTHODONTIC COMPOSITE RESINS ON HUMAN ORAL FIBROBLASTS. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 15(2), 31-38. SID. https://sid.ir/paper/35872/en

Vancouver: Copy

SADEGHIAN SOUSAN, NASRESFAHANI MOHAMMAD HOSSEIN, VATANI M.. IN VITRO BIOCOMPATIBILITY OF THREE ORTHODONTIC COMPOSITE RESINS ON HUMAN ORAL FIBROBLASTS. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2007;15(2):31-38. Available from: https://sid.ir/paper/35872/en

IEEE: Copy

SOUSAN SADEGHIAN, MOHAMMAD HOSSEIN NASRESFAHANI, and M. VATANI, “IN VITRO BIOCOMPATIBILITY OF THREE ORTHODONTIC COMPOSITE RESINS ON HUMAN ORAL FIBROBLASTS,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 15, no. 2, pp. 31–38, 2007, [Online]. Available: https://sid.ir/paper/35872/en

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