Evaluation of the effect of liposomal miR-143 on cell viability and antiapoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers

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BARZEGARI FIROUZABADI FATEMEH; ORYAN SHAHRBANOO; Sheikhha Mohammad Hassan; KALANTAR SEYED MEHDI; JAVED AMENEH

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2018 | Volume:26 | Issue:6 Page(s): 542-552

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Title

Evaluation of the effect of liposomal miR-143 on cell viability and antiapoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers

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Keywords

miR-143Q1

LipofectaminQ1

Bcl-2Q1

OsteosarcomaQ2

Abstract

Introdution: The present study was conducted to evaluate the effect of miR-143 on the expression of Bcl2 gene at the level of mRNA, cell cytotoxicity in the SaOs-2 cell line (Osteosarcoma) and Hum 63 (Human primary bone cells). Methods: In this experimental study, miR-143 was introduced into the SaOs-2 and Hum 63 cell by the lipofectam 2000 system. Then, the effects of miR-143 on cytotoxicity (72 hours) and expression of Bcl2 gene were measured by the MTT tests and Real Time Pcr protocols, respectively. Results: The results showed that the Lipofectamine 2000 system had no toxicity in both Hum63 and SaOs2 cell lines by itself. SaOs2 cancer cells were more susceptible to miR-143 and Lipo / miR-143 compared to Hum63 cells. The highest reduction in SaOs2 cells was observed after the treatment with Lipo / miR-143 (p <0. 05). Furthermore, according to the results, transfection of miR-143 by Lipofectamine 2000 into the cell, compared to cell-treated alone by miR-143, significantly reduced Bcl2 expression (p <0. 05). Conclusion: The lipoprotein 2000 system can be effective in absorbing miR-143 into the cells. By targeting various cellular pathways, including the expression level of the Bcl2 gene, miR-143 can be effective in the treatment of bone cancer (Osteosarcoma).

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APA: Copy

BARZEGARI FIROUZABADI, FATEMEH, ORYAN, SHAHRBANOO, Sheikhha, Mohammad Hassan, KALANTAR, SEYED MEHDI, & JAVED, AMENEH. (2018). Evaluation of the effect of liposomal miR-143 on cell viability and antiapoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 26(6 ), 542-552. SID. https://sid.ir/paper/36052/en

Vancouver: Copy

BARZEGARI FIROUZABADI FATEMEH, ORYAN SHAHRBANOO, Sheikhha Mohammad Hassan, KALANTAR SEYED MEHDI, JAVED AMENEH. Evaluation of the effect of liposomal miR-143 on cell viability and antiapoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;26(6 ):542-552. Available from: https://sid.ir/paper/36052/en

IEEE: Copy

FATEMEH BARZEGARI FIROUZABADI, SHAHRBANOO ORYAN, Mohammad Hassan Sheikhha, SEYED MEHDI KALANTAR, and AMENEH JAVED, “Evaluation of the effect of liposomal miR-143 on cell viability and antiapoptotic gene expression of Bcl-2 in human osteosarcoma cells for the treatment of metastatic cancers,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 26, no. 6 , pp. 542–552, 2018, [Online]. Available: https://sid.ir/paper/36052/en

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