Comparison of the Toxic Effects of Hydroalcoholic Extract of Glaucium flavum on Glioblastoma and Non-Cancer Nerve Cells

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

HOVEIZI E.; KALANTARI M.; HUSHMANDI K.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Armaghane Danesh Year:2020 | Volume:24 | Issue:6 (137) Page(s): 1073-1086

Download Full-Text

Persian Verion

View:

329

Download:

Cites:

Information Journal Paper

Title

Comparison of the Toxic Effects of Hydroalcoholic Extract of Glaucium flavum on Glioblastoma and Non-Cancer Nerve Cells

Author(s)

HOVEIZI E. | KALANTARI M. | HUSHMANDI K. | Issue Writer Certificate

Keywords

GlioblastomaQ2

Glacium flavumQ1

Neuronal precursor cellQ1

Cell viabilityQ1

Abstract

Background & aim: Glioblastoma tumors are one of the most invasive brain tumors of neural tissue origin. The low success rates of these types of tumors require the search for new treatment options. Glaucium flavum is widely used in the pharmaceutical industry because of its high content of alkaloids. The aim of this study was to determine and evaluate the toxic effects of hydroalcoholic extract of Glaucium flavum Glioblastoma and non-cancerous nerve cells. Methods: the present research was an experimental study carried out in the cell culture research laboratory of Shahid Chamran University of Ahvaz in 2019. A pregnant rat was used in this study for cell isolation. The Glaucium flavum extract was first extracted and nerve progenitor cells then extracted from the brain cortex of 17-day-old pregnant mouse embryos by enzymatic digestion. Cells were then treated with Glaucium flavum at concentrations of 100, 300, 500, and 1000 μ g/ml for 24 and 72 h. After treatment, Cell viability was assessed by MTT photometric method and cell morphology was evaluated by DAPI fluorescent staining. Data were analyzed using one-way ANOVA. Results: Morphological changes were observed in the cells treated with IC50. Evaluation of cell survival by MTT assay showed that the Glaucium flayum extract significantly decreased the survival of C118 cells as well as neural neurospheres (NCs) at both 24 and 72 h intervals. The severity of Glaucium flayum extract cytotoxicity on Glioblastoma cells was higher than the neurospheres and showed a significant difference (p <0. 05). The results of Cell viability also showed that the concentration of 300 and 500 μ g/ml of the Glaucium flayum extract was IC50 for C118 and NCs cells respectively. Conclusion: It was concluded that natural products such as Glaucium flayum extract with pharmacological and biochemical properties had antioxidant and anti-inflammatory properties and have less adverse effects on non-cancerous brain cells than in non-cancerous cells.

Cites

No record.

References

Cite

APA: Copy

HOVEIZI, E., KALANTARI, M., & HUSHMANDI, K.. (2020). Comparison of the Toxic Effects of Hydroalcoholic Extract of Glaucium flavum on Glioblastoma and Non-Cancer Nerve Cells. ARMAGHAN DANESH, 24(6 (137) ), 1073-1086. SID. https://sid.ir/paper/361034/en

Vancouver: Copy

HOVEIZI E., KALANTARI M., HUSHMANDI K.. Comparison of the Toxic Effects of Hydroalcoholic Extract of Glaucium flavum on Glioblastoma and Non-Cancer Nerve Cells. ARMAGHAN DANESH[Internet]. 2020;24(6 (137) ):1073-1086. Available from: https://sid.ir/paper/361034/en

IEEE: Copy

E. HOVEIZI, M. KALANTARI, and K. HUSHMANDI, “Comparison of the Toxic Effects of Hydroalcoholic Extract of Glaucium flavum on Glioblastoma and Non-Cancer Nerve Cells,” ARMAGHAN DANESH, vol. 24, no. 6 (137) , pp. 1073–1086, 2020, [Online]. Available: https://sid.ir/paper/361034/en

Related Journal Papers