Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BARZEGARI FIROUZABADI FATEMEH; ORYAN SHAHRBANOO; SHEIKHHA MOHAMMAD HASAN; KALANTAR SEYED MEHDI; JAVED AMENEH

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2018 | Volume:26 | Issue:3 Page(s): 275-285

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

381

Download:

Cites:

Information Journal Paper

Title

Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer

Author(s)

Keywords

miRNAQ1

NanocompositeQ1

Gene therapyQ2

Cell cytotoxicityQ2

Abstract

Introdution: The aim of this study was to investigate the physico-chemical properties, surface charge and cytotoxicity of Nanocomposite formulations for the design of lipid nano-systems with maximum loading for nucleic acids in bone marrow cancer. Methods For this study, six types of liposomes with different compositions were synthesized using DPPC, cholesterol, DSPE-mPEG (2000) and DOTAP. Then, nanosystems were evaluated for particle size, surface charge, cytotoxicity and loading rate of miRNA in two MG-63 and SaOs2 cell lines, which are human cell bone marrow cell lines. Results: All nano-systems were monodisperse. Among the systems, the F6 formula has the lowest toxicity and the highest load of miRNA-143, due to the presence of a suitable amount of DOTAP and PEG phospholipid in the liposome structure. Conclusion: Liposomal formulation with a suitable percentage of DOTAP cationic phospholipid can be used as a successful carrier for the transport of miRNA in the Gene therapy process for the treatment of various cancers, especially metastatic types.

Cites

No record.

References

No record.

Cite

APA: Copy

BARZEGARI FIROUZABADI, FATEMEH, ORYAN, SHAHRBANOO, SHEIKHHA, MOHAMMAD HASAN, KALANTAR, SEYED MEHDI, & JAVED, AMENEH. (2018). Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 26(3 ), 275-285. SID. https://sid.ir/paper/36212/en

Vancouver: Copy

BARZEGARI FIROUZABADI FATEMEH, ORYAN SHAHRBANOO, SHEIKHHA MOHAMMAD HASAN, KALANTAR SEYED MEHDI, JAVED AMENEH. Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;26(3 ):275-285. Available from: https://sid.ir/paper/36212/en

IEEE: Copy

FATEMEH BARZEGARI FIROUZABADI, SHAHRBANOO ORYAN, MOHAMMAD HASAN SHEIKHHA, SEYED MEHDI KALANTAR, and AMENEH JAVED, “Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 26, no. 3 , pp. 275–285, 2018, [Online]. Available: https://sid.ir/paper/36212/en

Related Journal Papers