Effect of curcumin and sorafenib on AKT gene expression in KG1 and U937 cell lines

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Pazahr R.; Mohammadi Kian M.; ALI ASGARI E.; MOHAMMADI S.; ROSTAMI SH.; Chahardolii B.; BABAKHANI D.; NIKBAKHT M.

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Journal: The Scientific Journal of Iranian Blood Transfusion Year:2020 | Volume:17 | Issue:2 Page(s): 113-125

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Title

Effect of curcumin and sorafenib on AKT gene expression in KG1 and U937 cell lines

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Keywords

Acute Myeloid LeukemiaQ3

CurcuminQ1

SorafenibQ1

Abstract

Background and Objectives Acute Myeloid Leukemia is a heterozygous hematologic malignancy that is manifested by the accumulation of hematopoietics stem cells in peripheral blood and bone marrow. Anticancer effects and cryotoxic activity of Curcumin have been proven frequently in many cancers. Sorafenib is known as an inhibitor of angiogenesis which prevent cells’ survival. In the present study, the effects of Curcumin and Sorafenib and their combination were evaluated on AML cells. Materials and Methods In this experimental study, U937 and KG-1 cells were treated with different concentration of Curcumin and Sorafenib and their combination. MTT assay was applied to assess the viability of cells. Percentage of apoptotic cells was evaluated by annexin PI staining. Also Real Time PCR was performed to investigte the level of AKT mRNA expression. Results Our data showed that the percentage of apoptotic cells significantly increased in response to treatment with Curcumin (40μ M in KG-1 and U937 cell lines), Sorafenib (5 μ M and 7 μ M in U937 and KG-1, respectively) and their combination. Moreover, the mRNA level of AKT gene was downregulated in KG-1 and U937 cells. Conclusions Our results suggest that combination of Curcumin and Sorafenib could lead to promote apoptosis. Furthurermore, downregulation of AKT gene shows that these agents can be considered as effective agents on AML cells.

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References

Regulation of the PI3K/Akt pathway during decidualization of endometrial stromal cells

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APA: Copy

Pazahr, R., Mohammadi Kian, M., ALI ASGARI, E., MOHAMMADI, S., ROSTAMI, SH., Chahardolii, B., BABAKHANI, D., & NIKBAKHT, M.. (2020). Effect of curcumin and sorafenib on AKT gene expression in KG1 and U937 cell lines. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), 17(2 ), 113-125. SID. https://sid.ir/paper/363165/en

Vancouver: Copy

Pazahr R., Mohammadi Kian M., ALI ASGARI E., MOHAMMADI S., ROSTAMI SH., Chahardolii B., BABAKHANI D., NIKBAKHT M.. Effect of curcumin and sorafenib on AKT gene expression in KG1 and U937 cell lines. THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON)[Internet]. 2020;17(2 ):113-125. Available from: https://sid.ir/paper/363165/en

IEEE: Copy

R. Pazahr, M. Mohammadi Kian, E. ALI ASGARI, S. MOHAMMADI, SH. ROSTAMI, B. Chahardolii, D. BABAKHANI, and M. NIKBAKHT, “Effect of curcumin and sorafenib on AKT gene expression in KG1 and U937 cell lines,” THE SCIENTIFIC JOURNAL OF IRANIAN BLOOD TRANSFUSION ORGANIZATION (KHOON), vol. 17, no. 2 , pp. 113–125, 2020, [Online]. Available: https://sid.ir/paper/363165/en

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