Frequency of CTX-M-1 Gene in Escherichia coli Isolates of ESBL-Producing Enzyme in Clinical Samples by Polymerase Chain Reaction (PCR) in Yasuj

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Khishvand Z.; HOSSEINI M.; Khoram Rooz S.S.; SHARIFI A.; KHOSRAVANI S.A.M.

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Journal: Armaghane Danesh Year:2020 | Volume:24 | Issue:6 (137) Page(s): 1116-1126

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Title

Frequency of CTX-M-1 Gene in Escherichia coli Isolates of ESBL-Producing Enzyme in Clinical Samples by Polymerase Chain Reaction (PCR) in Yasuj

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Keywords

E.coliQ2

ESBLQ2

PCRQ2

CTXM1Q2

Abstract

Background & aim: Resistance to β-lactam antibiotics in the clinical isolates, in most cases is caused by β-lactamase enzymes. In recent years, The incidence of broad-spectrum β-lactamase enzymes (ESBLs) among clinical isolates especially E. coli is greatly increased, since the β-lactamase have several subfamilies, using universal primers designed to detect the following complete families could be useful. β-lactamase producing enzymes (ESBLs) of E. coli has created many problems for patients. β-lactamase CTX-M-1 gene is the cause of resistance. The aim of this study was to evaluate CTX-M-1gene in E. coli. Methods: In this practical study, susceptibility of isolated bacteria to 13 antibiotics were indicated by disk diffusion method according to CLSI guidelines and strains were analyzed for the presence of widespread β-lactamase enzymes via two-disc synergy method. Thus, the prevalence of CTX-M1 ESBL gene samples were determined using PCR and the data were analyzed using ANOVA. Results: A total of 200 isolates of E. coli were isolated. The presence of CTX-M-1 gene were also isolated using the PCR method. From 200 strains studied, 62 (31%), of strains produced ESBL. After PCR processing of 62 produced ESBL, 43 isolates (69. 4%) were identified as CTX-M-1 genes. Also, antibiotic susceptibility test showed the highest percentage of resistance to Cotrimoxazole antibiotic (50%) and the lowest antibiotic resistance to imipenem (0%). Conclusion: The results of this study showed the high percentage of β-lactamase resistance among of E. coli strains. This is a serious public hazard that should be pointed out to measures for preventing this hazard. Considering the sensitivity of the studied beta-lactam resistant isolates and isolates in Iran to imipenem, a carbapenem with a non-beta-lactam antibiotic is recommended for the treatment of nosocomial infections caused by these strains.

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APA: Copy

Khishvand, Z., HOSSEINI, M., Khoram Rooz, S.S., SHARIFI, A., & KHOSRAVANI, S.A.M.. (2020). Frequency of CTX-M-1 Gene in Escherichia coli Isolates of ESBL-Producing Enzyme in Clinical Samples by Polymerase Chain Reaction (PCR) in Yasuj. ARMAGHAN DANESH, 24(6 (137) ), 1116-1126. SID. https://sid.ir/paper/363252/en

Vancouver: Copy

Khishvand Z., HOSSEINI M., Khoram Rooz S.S., SHARIFI A., KHOSRAVANI S.A.M.. Frequency of CTX-M-1 Gene in Escherichia coli Isolates of ESBL-Producing Enzyme in Clinical Samples by Polymerase Chain Reaction (PCR) in Yasuj. ARMAGHAN DANESH[Internet]. 2020;24(6 (137) ):1116-1126. Available from: https://sid.ir/paper/363252/en

IEEE: Copy

Z. Khishvand, M. HOSSEINI, S.S. Khoram Rooz, A. SHARIFI, and S.A.M. KHOSRAVANI, “Frequency of CTX-M-1 Gene in Escherichia coli Isolates of ESBL-Producing Enzyme in Clinical Samples by Polymerase Chain Reaction (PCR) in Yasuj,” ARMAGHAN DANESH, vol. 24, no. 6 (137) , pp. 1116–1126, 2020, [Online]. Available: https://sid.ir/paper/363252/en

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