THE FREQUENCY OF EXTENDED SPECTRUM BETA LACTAMASE AND CTX M-I OF ESCHERICHIA COLI ISOLATED FROM THE URINE TRACT INFECTION OF PATIENTS BY PHENOTYPIC AND PCR METHODS IN THE CITY OF KHOY IN IRAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SHARIFI YAZDI M.K.; AZARSA M.; SHIRAZI M.H.; RASTEGAR LARI A.; OWLIA P.; FALLAH MEHRABADI J.; MOLLA AGHAMIRZAEI H.; SABBAGHI A.; SHAMKANI F.; MOBASSERI G.; BAKHTIARI R.; SOLTAN DALLAL M.M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Advances in Medical and Biomedical Research Year:2012 | Volume:19 | Issue:77 Page(s): 53-61

Download Full-Text

Persian Verion

View:

917

Download:

Cites:

Information Journal Paper

Title

THE FREQUENCY OF EXTENDED SPECTRUM BETA LACTAMASE AND CTX M-I OF ESCHERICHIA COLI ISOLATED FROM THE URINE TRACT INFECTION OF PATIENTS BY PHENOTYPIC AND PCR METHODS IN THE CITY OF KHOY IN IRAN

Author(s)

Keywords

E.COLIQ2

EXTENDED SPECTRUM BETA LACTAMASEQ2

CTX-M-IQ2

ANTIMICROBIAL RESISTANCEQ2

Abstract

Background and Objective: The production of EXTENDED SPECTRUM BETA LACTAMASEs (ESBLs) by Escherichia coli is the main cause of resistance to Cephalosporins. In the past decade, CTX-M enzymes have become the most prevalent ESBLs in Europe, Canada, and Asia. In this study, the frequency of ESBLproducing E.COLI and molecular detection of the CTX-M-I group was investigated.Materials and Methods: A total of 400 urine samples were collected from both hospitalized and out-patients in Khoy’s hospitals between November 2009 and April 2010. Out of these samples, 188 were identified as E.COLI by standard biochemical tests. The antibiotic Susceptibility tests to 10 antibiotics were performed by the-disk-agar diffusion (DAD) method. ESBL production was screened by phenotypic test that including disk diffusion agar and combined disk as recommended by the Clinical and Laboratory Standards Institute (CLSI). Screened isolates were investigated by PCR assay for detection of CTX-M-I group genes.Results: The results show that out of 188 E.COLI isolates identified, 56 (29.8%) was producing ESBls by phenotypic test. All isolates were sensitive to imipenem. Overall, 49 (87.5%) isolates were confirmed as CTX-M-I producer by PCR.Conclusion: The results of this study showed that about 30% of the identified E.COLI was producing ESBl. Therefore, we recommend using molecular methods in such researches.

Cites

MOLECULAR ANALYSIS OF GENE FREQUENCIES OF TEM, CTX-M AND SHV IN BETA-LACTAM ANTIBIOTIC-RESISTANT STRAINS OF E. COLI ISOLATED FROM URINARY TRACT INFECTIONS IN YASUJ HOSPITALS

References

No record.

Cite

APA: Copy

SHARIFI YAZDI, M.K., AZARSA, M., SHIRAZI, M.H., RASTEGAR LARI, A., OWLIA, P., FALLAH MEHRABADI, J., MOLLA AGHAMIRZAEI, H., SABBAGHI, A., SHAMKANI, F., MOBASSERI, G., BAKHTIARI, R., & SOLTAN DALLAL, M.M.. (2012). THE FREQUENCY OF EXTENDED SPECTRUM BETA LACTAMASE AND CTX M-I OF ESCHERICHIA COLI ISOLATED FROM THE URINE TRACT INFECTION OF PATIENTS BY PHENOTYPIC AND PCR METHODS IN THE CITY OF KHOY IN IRAN. JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH, 19(77), 53-61. SID. https://sid.ir/paper/61096/en

Vancouver: Copy

SHARIFI YAZDI M.K., AZARSA M., SHIRAZI M.H., RASTEGAR LARI A., OWLIA P., FALLAH MEHRABADI J., MOLLA AGHAMIRZAEI H., SABBAGHI A., SHAMKANI F., MOBASSERI G., BAKHTIARI R., SOLTAN DALLAL M.M.. THE FREQUENCY OF EXTENDED SPECTRUM BETA LACTAMASE AND CTX M-I OF ESCHERICHIA COLI ISOLATED FROM THE URINE TRACT INFECTION OF PATIENTS BY PHENOTYPIC AND PCR METHODS IN THE CITY OF KHOY IN IRAN. JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH[Internet]. 2012;19(77):53-61. Available from: https://sid.ir/paper/61096/en

IEEE: Copy

M.K. SHARIFI YAZDI, M. AZARSA, M.H. SHIRAZI, A. RASTEGAR LARI, P. OWLIA, J. FALLAH MEHRABADI, H. MOLLA AGHAMIRZAEI, A. SABBAGHI, F. SHAMKANI, G. MOBASSERI, R. BAKHTIARI, and M.M. SOLTAN DALLAL, “THE FREQUENCY OF EXTENDED SPECTRUM BETA LACTAMASE AND CTX M-I OF ESCHERICHIA COLI ISOLATED FROM THE URINE TRACT INFECTION OF PATIENTS BY PHENOTYPIC AND PCR METHODS IN THE CITY OF KHOY IN IRAN,” JOURNAL OF ADVANCES IN MEDICAL AND BIOMEDICAL RESEARCH, vol. 19, no. 77, pp. 53–61, 2012, [Online]. Available: https://sid.ir/paper/61096/en

Related Journal Papers