Jundishapur Scientific Medical Journal
Year:2019 | Volume:18 | Issue:5|Papers Count:10

Background and Objectives: It is also known that diseases may start in the utero stage of development. Pregnancy is an important part of women's life. Various aspects including physical activity can affect different indicators of child health in adulthood. One of the common and growing diseases is vascular disease, that eNOS, BCL-2 and BAX are related indices. The aim of this study was to investigate the effect of aerobic exercise before and during pregnancy in adult males offsprings of Sprague Dawley rats on the expression of eNOS, BCL-2 and BAX genes in their femoral artery tissues. Materials and Methods: Twenty female Sprague Dawley rats (221 ± 10. 85g weight) were randomly divided into four groups: 1-exercise during pregnancy (TP), 2-exercise before and during pregnancy(Tbp), 3-exercise before pregnancy (Tb), 4-no exercise control (C). Mice performed moderate-intensity aerobic exercise before and during pregnancy. Tissue from the femoral artery of 8-week-old offspring rats was used to evaluate eNOS, BCL-2, BAX and BAX / BCL-2 gene expression. Results: Aerobic training before and during pregnancy, especially at both stages, resulted in a significant increase in eNOS and BCL-2 gene expression and a significant decrease in BAX and BAX/ BCL-2 gene expression. Conclusion: Based on these results, the present study showed that moderate-intensity aerobic exercise can make positive changes in factors related to vascular congestion in male offspring rats in which their mothers performed moderate-intensity aerobic exercises before and during their pregnancy.

Background and Objectives: Pain is a common complication after heart surgery that can cause problems for patients. This study aimed to investigate the effect of Holy Quran voice on pain and hemodynamic indices after cardiac surgery. Subjects and Methods: This single-blind randomized clinical trial was performed on heart surgery patients admitted to Golestan Hospital in Ahvaz in 2018. In the intervention group, after extubation, two times each day, for ten minutes, the voice of the Quran was broadcasted. For the control group, the usual care was continued. Data collection tools consisted of demographic and clinical and visual analog scale (VAS) as well as hemodynamic indices including systolic blood pressure, diastolic blood pressure, and heart rate. Data were analyzed by SPSS software version 22 using Chi-square, independent t-test and repeated ANOVA test. Results: There was no significant difference between qualitative and quantitative variables between the two groups, so the two groups were similar. Repeated ANOVA test showed that the use of Holy Quran voice was effective in reducing the pain intensity of patients (P <0. 001). Also, the results showed the positive effect of Holy Quran voice on systolic blood pressure (P<0. 001) and no significant effect on diastolic pressure and heart rate. Conclusion: The results of the study showed that the Holy Quran voice is effective in reducing pain and systolic blood pressure in patients after open-heart surgery. Therefore, this method can be used as non-invasive nursing care.

Background and Objective: There is a close relationship between the pathogenesis of obesity and the metabolic complications associated with secretion of some adipokines. The aim of this study was to evaluate the effect of rope jumping training on the secretion of vaspin, apelin and omentin-1 adipokines in obese adolescents. Subjects and Methods: In this semi-experimental study, 20 fat boys (mean age of 16± 1. 9 years, height 172± 9. 6 cm, weight 88. 6± 13. 2 kg) were selected from among volunteers and randomly placed in homogeneous groups, training group (n=10) and control group (n=10). The rope jumping training protocol was based on a six-week/five days/forty minutes per session. Blood samples and body composition measurements were measured before and after the protocol for measuring variables and measured using standard instruments and kits. Independent and dependent t-test was used for statistical analysis and significance level <0. 05. Results: At baseline, there were no statistically significant differences in age, weight, and BMI between the two groups. In the training group, serum apelin level (P=0. 002) and vaspin (P=0. 011) at the end of study compared with the beginning of the study, and the levels of omentin-1 (P=0. 001) increased significantly. Also, there were significant differences in the values of all variables between the two groups. Conclusion: The results of this study showed that rope jumping as a training method can be helpful in reducing vaspine and apelin adipokines and increasing omentin-1 in obese adolescents, and in this regard, improve the metabolic status of these individuals.

Background & Aim: The aim of this research was based on increasing of the quantity, quality and effectiveness of this vaccine, protection of personnel and cost-benefit of production. In this regard two diphtheria toxin separation methods (Separator and Filter press) were compared. Methods: Corynebacterium diphtheria (PW-8/CN-2000) was cultivated in 350L fermentor and half of the cultivation toxin clarified by Seitz filter press method and diatomaceous earth as filter aid and the rest was clarified by separator. Three sterile samples (50 mL) of each three produced batches were assessed by minimum lethal dose (MLD), specific toxicity test (STT), toxin flocculation titer (Lf, Kf – Ramon flocculation test) and SDS– PAGE. Other parameters like time consumption for toxin clarification with specific volume, quantity, product clarification, volume and clarification of product in two methods compared. Results: The results confirmed that the separator clarification method was more advantageous to filter press. Also, volume and transparency of the final product after clarification by separator were better than Filter press method. In addition, the separation time and costs used in the separator method were much less than Filter press method. Conclusion: In conclusion, use of separator clarification method was suitable alternative to current filter press method in terms of quantity and quality of diphtheria vaccine production.

Background and Objective: The aim of the present study was to evaluate the effect of melatonin supplementation on HSP60 and HSP70 gene expression changes following high intensity anaerobic exercise in male rats after ischemia-reperfusion. Materials and Methods: Twenty four male Wistar rats weighing approximately 200-250g were divided into 4 groups of 6 each: study groups including control, melatonin, anaerobic training, and anaerobic+melatonin training. The anaerobic exercise protocol was performed for one month, while the melatonin anaerobic training group underwent melatonin gavage during the exercise. At the end of one month, rats were injected with isoprenaline after two days of rest to induce ischemia-reperfusion. After two days the rats were killed, their left ventricle removed and the expression levels of HSP60 and HSP70 genes were measured by RNA extraction and Real Time technique. Results: Results showed that melatonin consumption decreased HSP60 and increased HSP70 apoptotic gene expression after anaerobic exercise (P <0. 05). Conclusion: The results of this study showed that intake of melatonin concurrent with intensive anaerobic exercise reduce the volume of myocardial apoptosis after ischemia-reperfusion-induced ischemia-reperfusion.

Venous and arterial thrombosis is uncommon in children and usually occurs in cases with central venous catheter, immobility, heart disease, trauma, cancer, surgery, infection, dehydration, shock, obesity, nephrotic syndrome, antiphospholipid antibody syndrome and coagulation disorders. Here a case report of an 8-year-old boy is reported who was referred with pain in the legs for 2 months before admission, morning headache, and vomiting for 3 weeks and cyanosis of the first and second right toes a week before admition. In the initial laboratory tests, the patient had only normochromic, normocytic anemia. In color doppler ultrasonography and computed tomography angiography, thrombosisin the right popliteal artery was reported, which was extended to proximal posterior of right tibialis artery. The patient was treated with aspirin, but developed pulmonary thrombosis and pancytopenia during admission. Bone marrow aspiration confirmed promyelocytic leukemia. Patient expired prior to chemotherapy due to extensive intracerebral hemorrhage.

Background and Objective: It has been shown that exercise can improve sleep quality, sleepiness, and quality of life in normal weight people. However, it is not clear whether similar beneficial effects can be gained in patients with multiple sclerosis (MS). The objective of this study was to investigate the effect of exercise training different genders and weights in MS patients. Subjects and Methods: Thirty-six persons with MS were recruited to participate in aerobic interval exercise training based on body weight status and gender, and divided into four groups include normal weight-male, normal weight-female, overweight-male and overweight-female. The exercise included 8-weeks of interval exercise at 60-75% Wattpeak. Sleep quality, sleepiness and quality of life were measured before and after the exercise training program. Results: All indicators showed significant improvement after interval exercise training. There were significant weight effects for improvement quality of sleep and life during exercise training. There were no significant gender and weight-gender interaction effects for improvement quality of sleep and life during exercise training. No significant weight, gender and interaction effect was found for sleepiness indicator during exercise training. Weight loss significantly associated with improvement of quality of life and sleep. Conclusion: The results confirm that exercise is an effective therapeutic method for improving quality of life and sleep, which dependent of body composition in people with MS.

Background and Objective: The aim was to compare the serum levels of vitamin D and uric acid and insulin resistance in obese subject with and whithout metabolic syndrom in Ahvaz. Subjects and Methods: A total of 86 obese (BMI>30) Diabetes Clinic and had the inclusion criteria were recruited in this study. They were divided in two groups: case (obese with metabolic syndrome) and control (obese without any signs of metabolic syndrome). Data were analyzed by SPSS software version 22. Results: Vitamin D deficiency was detected in 31 (54. 3%) obese subjects with metabolic syndrome and 14 (48. 2%) obese subjects without metabolic syndrome. There was no significant difference in serum levels of vitamin D among obese and non-metabolic syndromes (P>0. 05). Insulin resistance in obese people with metabolic syndrome was significantly higher than obese people without metabolic syndrome (P <0. 05). Conclusion: Insulin resistance in obese subjects with metabolic syndrome appears to be more common in obese subjects without metabolic syndrome.

Background and Objective: Platinum-based chemotherapy drugs like cisplatin are widely used in the treatment of cancer. However, because of its high side effects, the use of these compounds is limited. All of today's efforts are to design new platinum drugs, as well as hybrid of gold and platinum, which have a similar profile with cisplatin but have less side effects and wide spectrum on different cancer types. Material and Method: In this study, the cytotoxic activity of heterobimetallic Pt(II)-Au(I) complexes on the cancer cell lines such as cervix (Hela), colon (SW1116), breast (MDA-MB-231) as well as normal breast (MCF-12A) cell line was evaluated using MTT method. Molecular docking studies of these compounds with DNA and thioredoxin reductase (TrxR), as targets for platinum and gold compounds, were conducted using AutoDock 4. 2 software. Using the comet assay, genotoxicity of the best cytotoxic compound was also evaluated. Results: In evaluating the cytotoxic activity, 1b and 2b complexes exhibited greater cytotoxicity than cisplatin. All compounds exhibited lower toxicity than cisplatin on normal breast cell line (MCF-12A). The interaction of complexes with DNA and TrxR using molecular docking showed their binding mode and binding site to these targets. The genotoxicity effect of these compounds was also confirmed by the comet assay. Conclusion: The results showed that these complexes have the ability to play a more effective role in the treatment of cancer by further biological studies.

Background and Objective: Kidney transplantation is a treatment of choice in most of the patients with end-stage of renal disease (ESRD) and its outcomes are far more favorable than hemodialysis. The patient and graft survival rates are important issues for these patients and results are different in various centers throughout the world. Analyzing renal transplantation data can help to evaluate the effectiveness of transplantation interventions. The objective of the present study was to determine the patient and graft survival rate after kidney transplantation during a period of 10 years among our transplanted patients in Khouzestan Province, Iran. Subjects and Methods: In this retrospective cohort study, all recipients of kidney transplantation including 275 patients (age 8-70 years) between 2008 and 2018 who had medical records in Golestan Hospital, Ahvaz, Iran, were included. Kaplan-Meier method was used to determine the survival rate and Cox regression model to define the hazard ratio. Statistical analysis was conducted by SPSS software version 22. Results: The one-year patients and graft survival rates were 95. 8% and 97. 6% respectively. The five-year patient and graft survival rates were 90. 4% and 96. 3% and ten-year patients and graft survival rates were 88. 2% and 92. 5% respectively. The incidence of diabetes was significantly associated with the patient survival rate (P=0. 0001) and pre-transplant dialysis was associated with graft survival (P=0. 025). In this study, the survival rate was independent of the type of donor and recipient characteristic, and only diabetes was an effective factor in predicting the survival rate of kidney transplant patients (OR=2. 86; CI: 1. 26-6. 50; P=0. 012). Conclusion: Results of this study demonstrated that 1, 5 and 10-year patients and graft survival rates were appropriate in the kidney transplant center of Khuzestan and shows remarkable improvement over the past years. Diabetes was a significant predictor of survival in recipients of kidney transplantation and this issue should be considered in order to increase the survival.