The Effects of Fibroblast and Macrophage Separated and Co-Culture Conditioned Medium on Sperm Parameters Disorders Induced by Testicular Torsion-Detorsion in Adult Rats

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MIRZAEI M.; SHAHROOZ R.; SHALIZAR JALALI A.; Sarrafzadeh Rezaei F.; MOSAFFA N.

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Journal: Journal of Advanced Biomedical Sciences Year:2019 | Volume:9 | Issue:3 Page(s): 1564-1576

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Title

The Effects of Fibroblast and Macrophage Separated and Co-Culture Conditioned Medium on Sperm Parameters Disorders Induced by Testicular Torsion-Detorsion in Adult Rats

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Keywords

Testicular torsion-detorsionQ1

FibroblastQ2

MacrophageQ2

Sperm qualityQ2

RatQ2

Abstract

Background & Objective: Ischemia and Reperfusion (I/R) lead to the production of excessive reactive oxygen species (ROS). In the present study, the protective effects of the cell culture conditioned medium (CM) on Sperm quality disorder induced by torsion-detorsion (T-D) in adult Rat’ s testis was evaluated. Materials & Methods: In this study, 42 adult Rats were divided into seven groups. 1-Normal control group treated without surgery. 2-Laparotomy group, surgery opeRated without T-D. 3-T-D group, surgery opeRated along with T-D. 4-T-D+DMEM group, after T-D Dulbecco's Modified Eagle's Medium (DMEM) has been injected. 5-7 groups included T-D+F, T-D+M, and T-D+FM, along with TD injected conditioned medium of Fibroblast, Macrophage, and Fibroblast-Macrophage co-culture respectively. All injections were made at a Rate of 10μ L once after T-D in the rete testis. After 35 days, Sperm quality parameters were evaluated and obtained data were compared between groups by oneway ANOVA statistic method and Tukey test post hoc at P<0. 05 level. Results: The experimental groups that received CM of the Fibroblast and Macrophage compared to groups T-D and T-D+DMEM showed amelioRative effects in comparison with T-D and T-D+DMEM groups. No amelioRative effects were observed in the T-D+FM group. Conclusion: The results of this study showed the high ability of supernatant of the sepaRate Fibroblast and Macrophage culture in improving sperm parameters after testicular T-D.

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APA: Copy

MIRZAEI, M., SHAHROOZ, R., SHALIZAR JALALI, A., Sarrafzadeh Rezaei, F., & MOSAFFA, N.. (2019). The Effects of Fibroblast and Macrophage Separated and Co-Culture Conditioned Medium on Sperm Parameters Disorders Induced by Testicular Torsion-Detorsion in Adult Rats. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 9(3 ), 1564-1576. SID. https://sid.ir/paper/366514/en

Vancouver: Copy

MIRZAEI M., SHAHROOZ R., SHALIZAR JALALI A., Sarrafzadeh Rezaei F., MOSAFFA N.. The Effects of Fibroblast and Macrophage Separated and Co-Culture Conditioned Medium on Sperm Parameters Disorders Induced by Testicular Torsion-Detorsion in Adult Rats. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2019;9(3 ):1564-1576. Available from: https://sid.ir/paper/366514/en

IEEE: Copy

M. MIRZAEI, R. SHAHROOZ, A. SHALIZAR JALALI, F. Sarrafzadeh Rezaei, and N. MOSAFFA, “The Effects of Fibroblast and Macrophage Separated and Co-Culture Conditioned Medium on Sperm Parameters Disorders Induced by Testicular Torsion-Detorsion in Adult Rats,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 9, no. 3 , pp. 1564–1576, 2019, [Online]. Available: https://sid.ir/paper/366514/en

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