Prevalence of Methicillin-resistant (MRSA) Gene in Staphylococcus aureus Isolates Expressing PantonValentin Leukocidin (pvl) Gene Recovered from Clinical Cases in Rasht Hospitals

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Pourghafar H.; AMIRMOZAFARI N.; ANVARI M.

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Journal: Journal of Advanced Biomedical Sciences Year:2020 | Volume:10 | Issue:1 Page(s): 2094-2102

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Title

Prevalence of Methicillin-resistant (MRSA) Gene in Staphylococcus aureus Isolates Expressing PantonValentin Leukocidin (pvl) Gene Recovered from Clinical Cases in Rasht Hospitals

Author(s)

Pourghafar H. | AMIRMOZAFARI N. | ANVARI M. | Issue Writer Certificate

Keywords

Staphylococcus aureusQ2

Panton Valentine LeucocidinQ1

mecAQ2

MRSAQ2

Abstract

Background & Objective: Panton-Valentine Leukocidin (PVL) is associated with strains of Staphylococcus aureus that produce a high level of virulence which is characterized by skin abscesses and acute necrosis. Materials & Methods: During a six-month treatment period, a total of 92 clinical isolates of Staphylococcus were obtained. Initial tests were performed using the Disk Diffusion Method and Methicillin resistance test determined by an Oxacillin disk. In order to elucidate the frequency detection of pvl gene, standard PCR was accomplished using specific primers and then pvl positive isolates were further analyzed for the presence of mecA gene by the use of specific primers in PCR. Result: In total, 18 isolates (19. 56%) were shown to be positive in terms of their carrying the pvl gene among which, 15 isolates (83. 33%) were MRSA, 3 (16. 66%) were MSSA, and 8 (44. 44%) were positive for mecA gene. Conclusion: Despite the existence of pvl genes in MRSA isolates, PVL toxin producing strains of Staphylococcus aureus are serious threats for health. So, it seems that achieving a rapid and repeatable method for medical center, will help the timely diagnosis and control of PVL-producing strains.

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APA: Copy

Pourghafar, H., AMIRMOZAFARI, N., & ANVARI, M.. (2020). Prevalence of Methicillin-resistant (MRSA) Gene in Staphylococcus aureus Isolates Expressing PantonValentin Leukocidin (pvl) Gene Recovered from Clinical Cases in Rasht Hospitals. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 10(1 ), 2094-2102. SID. https://sid.ir/paper/366644/en

Vancouver: Copy

Pourghafar H., AMIRMOZAFARI N., ANVARI M.. Prevalence of Methicillin-resistant (MRSA) Gene in Staphylococcus aureus Isolates Expressing PantonValentin Leukocidin (pvl) Gene Recovered from Clinical Cases in Rasht Hospitals. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2020;10(1 ):2094-2102. Available from: https://sid.ir/paper/366644/en

IEEE: Copy

H. Pourghafar, N. AMIRMOZAFARI, and M. ANVARI, “Prevalence of Methicillin-resistant (MRSA) Gene in Staphylococcus aureus Isolates Expressing PantonValentin Leukocidin (pvl) Gene Recovered from Clinical Cases in Rasht Hospitals,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 10, no. 1 , pp. 2094–2102, 2020, [Online]. Available: https://sid.ir/paper/366644/en

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