Detection of Clostridium Difficile Associated Diarrhea in Disease Based on Polymerase Chain Reaction and Bacterial Culture and Toxin A, B Frequency

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ZARGAR M.; JAVADI A.; MOROVVATI A.

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Journal: Journal of Advanced Biomedical Sciences Year:2019 | Volume:9 | Issue:3 Page(s): 1596-1604

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Title

Detection of Clostridium Difficile Associated Diarrhea in Disease Based on Polymerase Chain Reaction and Bacterial Culture and Toxin A, B Frequency

Author(s)

ZARGAR M. | JAVADI A. | MOROVVATI A. | Issue Writer Certificate

Keywords

diarrheaQ3

PCRQ2

ccd3 geneQ1

Clostridium difficileQ2

toxin AQ2

BQ2

Abstract

Background & Objective: Clostridium difficile is an obligate anaerobic, gram positive bacillus. The purpose of this study was to compare PCR technique and bacterial culture to assess the prevalence of Clostridium difficile infection in the samples of watery diarrhea. Materials & Methods: This cross-sectional qualitative study was performed on 68 samples of watery diarrhea in Qom province hospitals. All samples were cultured in the specialized CCFA Agar medium. Specific primers were applied for the PCR assay based on cdd3 gene. Based on this primer, PCR product for this gene must be 622bp. toxin A & B diagnosis based on specific primers and product must be 473 and 272 bp Results: In this study, the optimized PCR technique was determined to be bacterium in 16 samples. But bacteria diagnosis in 11 samples by culture method. 6 samples had toxin A and B, 4samples had toxin A and 3 samples had toxin B. Conclusion: Cytotoxicity method is gold standard for Clostridium difficile. Diagnosis of these bacteria is very important and PCR was found as an applicable, sensitive, and quick technique for the detection of Clostridium difficile in compromise culture medium method.

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APA: Copy

ZARGAR, M., JAVADI, A., & MOROVVATI, A.. (2019). Detection of Clostridium Difficile Associated Diarrhea in Disease Based on Polymerase Chain Reaction and Bacterial Culture and Toxin A, B Frequency. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, 9(3 ), 1596-1604. SID. https://sid.ir/paper/367416/en

Vancouver: Copy

ZARGAR M., JAVADI A., MOROVVATI A.. Detection of Clostridium Difficile Associated Diarrhea in Disease Based on Polymerase Chain Reaction and Bacterial Culture and Toxin A, B Frequency. JOURNAL OF ADVANCED BIOMEDICAL SCIENCES[Internet]. 2019;9(3 ):1596-1604. Available from: https://sid.ir/paper/367416/en

IEEE: Copy

M. ZARGAR, A. JAVADI, and A. MOROVVATI, “Detection of Clostridium Difficile Associated Diarrhea in Disease Based on Polymerase Chain Reaction and Bacterial Culture and Toxin A, B Frequency,” JOURNAL OF ADVANCED BIOMEDICAL SCIENCES, vol. 9, no. 3 , pp. 1596–1604, 2019, [Online]. Available: https://sid.ir/paper/367416/en

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