EVALUATION AND COMPARISON OF PHYSICOCHEMICAL PROPERTIES, CYTOTOXICITY AND THE ABILITY OF MIRNA LOADING OF DIFFERENT CATIONIC LIPOSOMES FOR GENE THERAPY APPLICATION

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NIKOONAHAD LOTFABADI NARGES; MOHSENI KOUCHESFEHANI HOMA; SHEIKHHA MOHAMMAD HASAN; KALANTAR SEYED MEHDI

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Journal: Journal of Shahid Sadoughi University of Medical Sciences Year:2017 | Volume:25 | Issue:6 Page(s): 444-456

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Title

EVALUATION AND COMPARISON OF PHYSICOCHEMICAL PROPERTIES, CYTOTOXICITY AND THE ABILITY OF MIRNA LOADING OF DIFFERENT CATIONIC LIPOSOMES FOR GENE THERAPY APPLICATION

Author(s)

NIKOONAHAD LOTFABADI NARGES | MOHSENI KOUCHESFEHANI HOMA | SHEIKHHA MOHAMMAD HASAN | KALANTAR SEYED MEHDI | Issue Writer Certificate

Keywords

MIRNAQ1

LIPOSOMEQ2

CYTOTOXICITYQ2

GENE THERAPYQ2

Abstract

Introduction: In the present study, various formulations of cationic LIPOSOMEs were designed and prepared using different cationic lipids. It was performed to assess the physicochemical properties, MIRNA loading ability and cellular toxicity rates of LIPOSOMEs in order to use in GENE THERAPY.Methods: Different cationic LIPOSOME formulations (F1-F4) containing various cationic lipids, DOTAP, DOTMA, DOAB and DDAB with DPPC, cholesterol and phospholipid DSPE-mPEG were synthesized. Prepared nanoparticles were evaluated in term of particle size, polydisparity index, surface charge and CYTOTOXICITY for 48 and 72 h in two cell lines. By using gel electrophoresis, the ability of synthesized cationic LIPOSOMEs to entrap MIRNA was also compared.Results: All formulations were mono-dispersed. The particle size in F1, which contained DOTAP was lower than others (F2-F4) and its surface charge was more than them. Cationic LIPOSOMEs based on DOTAP had no significant CYTOTOXICITY as compared to other formulations. Also, F1 formulation was more capable to entrap MIRNA than other formulas.Conclusion: DOTAP-based cationic LIPOSOMEs can be used efficiently in the GENE THERAPY process, especially for the transfer of MIRNA as a new therapeutic agent in cancer therapy.

Cites

Niosomes from Preparation to Application in Drug Delivery

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APA: Copy

NIKOONAHAD LOTFABADI, NARGES, MOHSENI KOUCHESFEHANI, HOMA, SHEIKHHA, MOHAMMAD HASAN, & KALANTAR, SEYED MEHDI. (2017). EVALUATION AND COMPARISON OF PHYSICOCHEMICAL PROPERTIES, CYTOTOXICITY AND THE ABILITY OF MIRNA LOADING OF DIFFERENT CATIONIC LIPOSOMES FOR GENE THERAPY APPLICATION. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, 25(6 ), 444-456. SID. https://sid.ir/paper/36748/en

Vancouver: Copy

NIKOONAHAD LOTFABADI NARGES, MOHSENI KOUCHESFEHANI HOMA, SHEIKHHA MOHAMMAD HASAN, KALANTAR SEYED MEHDI. EVALUATION AND COMPARISON OF PHYSICOCHEMICAL PROPERTIES, CYTOTOXICITY AND THE ABILITY OF MIRNA LOADING OF DIFFERENT CATIONIC LIPOSOMES FOR GENE THERAPY APPLICATION. JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2017;25(6 ):444-456. Available from: https://sid.ir/paper/36748/en

IEEE: Copy

NARGES NIKOONAHAD LOTFABADI, HOMA MOHSENI KOUCHESFEHANI, MOHAMMAD HASAN SHEIKHHA, and SEYED MEHDI KALANTAR, “EVALUATION AND COMPARISON OF PHYSICOCHEMICAL PROPERTIES, CYTOTOXICITY AND THE ABILITY OF MIRNA LOADING OF DIFFERENT CATIONIC LIPOSOMES FOR GENE THERAPY APPLICATION,” JOURNAL OF SHAHID SADOUGHI UNIVERSITY OF MEDICAL SCIENCES, vol. 25, no. 6 , pp. 444–456, 2017, [Online]. Available: https://sid.ir/paper/36748/en

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