THE INVESTIGATION ON THE SKIN GRAFT BY EPITHELIAL CELLS CULTURE

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SAFARI M.; NASR ELAHZADEH B.; SHAMSHIRI MORTEZA

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Journal: Koomesh Year:2000 | Volume:1 | Issue:3 Page(s): 35-40

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Title

THE INVESTIGATION ON THE SKIN GRAFT BY EPITHELIAL CELLS CULTURE

Author(s)

SAFARI M. | NASR ELAHZADEH B. | SHAMSHIRI MORTEZA | Issue Writer Certificate

Keywords

AUTOLOGUSQ3

CULTUREQ3

GRAFTQ3

SKINQ3

Abstract

Introduction: Due to high incidence of SKIN damages, finding new methods for SKIN transplantation is quite important. The aim of this study was to produce normal SKIN by CULTURE of epithelial cells and to examine its AUTOLOGUS GRAFT. Materials and Methods: 4 male albino rabbits (4 months) were used. At the first from one of the rabbits a piece of SKIN (6×6 cm) separated from back region and placed in MEM and FCS solution as a supporting surface. From another rabbit, a piece of SKIN (2×1 cm) separated and divided into small particles and placed on the derm of supporting surface. By two other rabbits, this process was accomplished once more.After preparation, it GRAFTed to the second rabbit. After 4 weeks the animal killed and the GRAFTed region separated and prepared for histological study by two staining methods. Results: The results show that in the GRAFTed region, epidermis is normal but outer layer (cornea) is thinner than normal SKIN. Histological studies have been shown that SKIN elements were decreased, sweat glands were not found, repair was started from margin of ulcer, rete ridges were little and short, collagen of derm was regulated and vessels were dilated. Conclusion: With this method, the donor SKIN can be expanded to several times and used for AUTOLOGUS SKIN GRAFT.

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APA: Copy

SAFARI, M., NASR ELAHZADEH, B., & SHAMSHIRI, MORTEZA. (2000). THE INVESTIGATION ON THE SKIN GRAFT BY EPITHELIAL CELLS CULTURE . KOOMESH, 1(3), 35-40. SID. https://sid.ir/paper/36844/en

Vancouver: Copy

SAFARI M., NASR ELAHZADEH B., SHAMSHIRI MORTEZA. THE INVESTIGATION ON THE SKIN GRAFT BY EPITHELIAL CELLS CULTURE . KOOMESH[Internet]. 2000;1(3):35-40. Available from: https://sid.ir/paper/36844/en

IEEE: Copy

M. SAFARI, B. NASR ELAHZADEH, and MORTEZA SHAMSHIRI, “THE INVESTIGATION ON THE SKIN GRAFT BY EPITHELIAL CELLS CULTURE ,” KOOMESH, vol. 1, no. 3, pp. 35–40, 2000, [Online]. Available: https://sid.ir/paper/36844/en

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