ULCERT REPAIR BY SPRAY OF EPITHELIAL STEM CELLS

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SAFARI MANOUCHEHR; GHAHARI L.; KHOUSHVAGHTI A.; NASIRI E.

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Journal: Anatomical Sciences Journal Year:2008 | Volume:6 | Issue:23 Page(s): 375-383

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Information Journal Paper

Title

ULCERT REPAIR BY SPRAY OF EPITHELIAL STEM CELLS

Author(s)

SAFARI MANOUCHEHR | GHAHARI L. | KHOUSHVAGHTI A. | NASIRI E. | Issue Writer Certificate

Keywords

AUTOLOGUEQ3

SKINQ3

RABBITQ3

CELL CULTUREQ2

STEM CELLSQ2

Abstract

Purpose: Separation, prolifration of STEM CELLS and repairing of injured parts using these cellsMaterials and Methods: This was a Lab-Experimental study. We used 6 male albino RABBITs. At first, under general anesthesia 5x5 cm2 full thickness SKIN from one of the RABBITs separated, washed using 70% alcohol and inserted in cold HBSS. Then it was cut into 4-6 mm pieces, washed again and incubated in Tripsin- EDTA 0.1% for 30 minutes at 37C. The cell suspention was then centrifuged and resuspended in DMEM culture. After 10 dayes the cells separated from the bed using Tripsin method. In experimental group the wounds were sprayed with 2 ml cultured keratinocytes cells and bandaged with vazeline. In control group just the SKIN was removed and the wounds healed withought cell spray. At the end of 4th week all rats were sacrificed, the repaired regions separated and studied with H&E and tricrom mason staining.Results: In cell studies, the colonies of STEM CELLS were visible using special staining. In Histological studies the epiderm of repaired wounds with cells were normal but the keratoid layers were thiner than normal SKIN. No sweat gland was observed. Other findings were: shorter finger nodes, regular collagen fiber in dermal layer and wider vesseles.Conclusion: This method of CELL CULTURE would repaire a wide region and obtain a normal SKIN in a little time.

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APA: Copy

SAFARI, MANOUCHEHR, GHAHARI, L., KHOUSHVAGHTI, A., & NASIRI, E.. (2008). ULCERT REPAIR BY SPRAY OF EPITHELIAL STEM CELLS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES, 6(23), 375-383. SID. https://sid.ir/paper/81562/en

Vancouver: Copy

SAFARI MANOUCHEHR, GHAHARI L., KHOUSHVAGHTI A., NASIRI E.. ULCERT REPAIR BY SPRAY OF EPITHELIAL STEM CELLS. JOURNAL OF IRANIAN ANATOMICAL SCIENCES[Internet]. 2008;6(23):375-383. Available from: https://sid.ir/paper/81562/en

IEEE: Copy

MANOUCHEHR SAFARI, L. GHAHARI, A. KHOUSHVAGHTI, and E. NASIRI, “ULCERT REPAIR BY SPRAY OF EPITHELIAL STEM CELLS,” JOURNAL OF IRANIAN ANATOMICAL SCIENCES, vol. 6, no. 23, pp. 375–383, 2008, [Online]. Available: https://sid.ir/paper/81562/en

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