EFFECTS OF SPEED AND TIME OF CENTRIFUGATION AND TIME OF FREEZING ON THE AMOUNT OF PRODUCED MICROPARTICLES FROM CONCENTRATES PLATELET

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MANOOCHEHRABADI TAHERE; SHARIFI ZOHREH; YARI FATEMEH; REZVANI BROUJENI EHLAM; MIRSHAFIE HAMIDEH; NIKOUGOFTAR ZARIF MAHIN; HASANNEJAD GHASEM

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Journal: Koomesh Year:2016 | Volume:17 | Issue:3 (59) Page(s): 686-691

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Title

EFFECTS OF SPEED AND TIME OF CENTRIFUGATION AND TIME OF FREEZING ON THE AMOUNT OF PRODUCED MICROPARTICLES FROM CONCENTRATES PLATELET

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Keywords

BLOOD PLATELETSQ2

CENTRIFUGATIONQ1

FREEZINGQ1

CELL-DERIVED MICROPARTICLESQ1

Abstract

Introduction: The main method of separating platelet microparticles (PMP) is based on the CENTRIFUGATION speed and time. Due to the high cost of determining the number of PMP via micro-particles (micro bead) and also the necessity of using an expensive device such as a flow cytometer, it seems that Bradford method would be rather an inexpensive, fast and efficient way to determine the concentration of PMP. Therefore, in this study the effect of different factors, such as speed and time of CENTRIFUGATION and time of FREEZING on the concentration of PMP in the platelet concentrates bags was studied.Materials and Methods: We studied two different speeds of CENTRIFUGATION for separating PRP. In the first protocol for preparation of PRP, the platelet bags were centrifuged at 1500g for 15min and in the second protocol; they were centrifuged at 5000g for the same duration. To evaluate the effect of time, microparticles were separated in 16000g for 20 and 2 min. To determine the concentration of PMP, Bradford method was used. To evaluation the effect of FREEZING, the PRP was prepared at 300g for 20 min, and then it was freezed in -80oc for five days. Flow cytometery analysis was performed for microparticles identification.Results: PMP concentrates with the 1500g CENTRIFUGATION speed showed higher concentration (P<0.05). There was not any significant difference in concentrations of PMPs in relation to the time of CENTRIFUGATION (2 and 20 min) (P<0.05). FREEZING the platelet bags led to higher PMP concentration in compare to the first day of experiment. Flow cytometry analysis showed that microparticles had platelet marker CD41, which represented their origin.Conclusion: The result of this study showed that the reduction of CENTRIFUGATION speed could produce higher levels of the microparticles. In addition, the time of separation in the final stage had no significant effect on PMP isolation. FREEZING could lead to higher PMP concentration.

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References

EFFECTS OF PLATELET MICROPARTICLES ON THE ACTIVATION OF B CELLS

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APA: Copy

MANOOCHEHRABADI, TAHERE, SHARIFI, ZOHREH, YARI, FATEMEH, REZVANI BROUJENI, EHLAM, MIRSHAFIE, HAMIDEH, NIKOUGOFTAR ZARIF, MAHIN, & HASANNEJAD, GHASEM. (2016). EFFECTS OF SPEED AND TIME OF CENTRIFUGATION AND TIME OF FREEZING ON THE AMOUNT OF PRODUCED MICROPARTICLES FROM CONCENTRATES PLATELET. KOOMESH, 17(3 (59)), 686-691. SID. https://sid.ir/paper/36866/en

Vancouver: Copy

MANOOCHEHRABADI TAHERE, SHARIFI ZOHREH, YARI FATEMEH, REZVANI BROUJENI EHLAM, MIRSHAFIE HAMIDEH, NIKOUGOFTAR ZARIF MAHIN, HASANNEJAD GHASEM. EFFECTS OF SPEED AND TIME OF CENTRIFUGATION AND TIME OF FREEZING ON THE AMOUNT OF PRODUCED MICROPARTICLES FROM CONCENTRATES PLATELET. KOOMESH[Internet]. 2016;17(3 (59)):686-691. Available from: https://sid.ir/paper/36866/en

IEEE: Copy

TAHERE MANOOCHEHRABADI, ZOHREH SHARIFI, FATEMEH YARI, EHLAM REZVANI BROUJENI, HAMIDEH MIRSHAFIE, MAHIN NIKOUGOFTAR ZARIF, and GHASEM HASANNEJAD, “EFFECTS OF SPEED AND TIME OF CENTRIFUGATION AND TIME OF FREEZING ON THE AMOUNT OF PRODUCED MICROPARTICLES FROM CONCENTRATES PLATELET,” KOOMESH, vol. 17, no. 3 (59), pp. 686–691, 2016, [Online]. Available: https://sid.ir/paper/36866/en

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