RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX: PCR METHOD USING INSERTION SEQUENCE 6110

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ROHANI M.; KHORSHIDI A.; MONIRI R.; TORFEH M.; ABDDOSHAH F.; SAFFARI M.; SHAJARI GH.R.; MOOSAVI GH.A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ) Year:2009 | Volume:67 | Issue:3 Page(s): 173-177

Download Full-Text

Persian Verion

View:

1,314

Download:

Cites:

Information Journal Paper

Title

RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX: PCR METHOD USING INSERTION SEQUENCE 6110

Author(s)

Keywords

MYCOBACTERIUM TUBERCULOSIS COMPLEXQ3

DETECTIONQ3

IS6110Q3

PCRQ3

Abstract

Background: Tuberculosis is an important cause of death in some countries. The world health organization estimates that if stronger measures are not taken up to control the prevalence of this disease, from2000 to 2020 a billion people will be infected by the bacterium. According to time consuming of common DETECTION methods of Mycobacterium tuberculosis such as culture, it is necessary to evaluate a rapid DETECTION tests such as PCR. Rapid diagnosis of tuberculosis may have profound effects in patients’care According to importance of rapid DETECTION and treatment of tuberculosis and for determine of sensitivity, specificity, positive predictive value and negative predictive value of PCR by using IS6110 this study was done in Kashan university of medical science.Methods: A total of 248 sputum samples from patients suspected of mycobacterial diseases were studied. DNA was extracted by boiling method. IS6110 PCR method by a specific pair of primers designed to amplify123bp and 245bp sequences of the insertion sequence, 6110, in the M. tuberculosis genome was used to analyze sputum samples.Results: 32 out of 248 (12.9%) of samples had positive culture. PCR yielded a sensitivity of93.8 % and specificity of 99.1% for the diagnosis of TB patients with TB confirmed by culture. There were two out of32 (6.3%) PCR-positive cases among the patients with non-TB disease.Conclusion: The findings of the present study indicate that Multiplex PCR may provide a faster method of detecting tuberculosis, thus enhancing diagnostic procedures and we conclude that the performance of anIS6110 PCR assay is valuable in the rapid diagnosis of tuberculosis.

Cites

No record.

References

No record.

Cite

APA: Copy

ROHANI, M., KHORSHIDI, A., MONIRI, R., TORFEH, M., ABDDOSHAH, F., SAFFARI, M., SHAJARI, GH.R., & MOOSAVI, GH.A.. (2009). RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX: PCR METHOD USING INSERTION SEQUENCE 6110. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 67(3), 173-177. SID. https://sid.ir/paper/37991/en

Vancouver: Copy

ROHANI M., KHORSHIDI A., MONIRI R., TORFEH M., ABDDOSHAH F., SAFFARI M., SHAJARI GH.R., MOOSAVI GH.A.. RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX: PCR METHOD USING INSERTION SEQUENCE 6110. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2009;67(3):173-177. Available from: https://sid.ir/paper/37991/en

IEEE: Copy

M. ROHANI, A. KHORSHIDI, R. MONIRI, M. TORFEH, F. ABDDOSHAH, M. SAFFARI, GH.R. SHAJARI, and GH.A. MOOSAVI, “RAPID DETECTION OF MYCOBACTERIUM TUBERCULOSIS COMPLEX: PCR METHOD USING INSERTION SEQUENCE 6110,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 67, no. 3, pp. 173–177, 2009, [Online]. Available: https://sid.ir/paper/37991/en

Related Journal Papers