ANTIGENICITY OF RECOMBINANT L7/L12 IN PATIENTS WITH BRUCELLOSIS

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ABTAHI HAMID; SALMANIAN ALI HATEF

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Journal: Koomesh Year:2012 | Volume:13 | Issue:2 (42) Page(s): 167-171

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Title

ANTIGENICITY OF RECOMBINANT L7/L12 IN PATIENTS WITH BRUCELLOSIS

Author(s)

ABTAHI HAMID | SALMANIAN ALI HATEF | Issue Writer Certificate

Keywords

BRUCELLA ABORTUSQ3

L7/L12 RECOMBINANT PROTEINQ2

BRUCELLOSISQ3

BLOTTINGQ3

WESTERNQ3

ESCHERICHIA COLIQ3

Abstract

Introduction: Immune response to recombinant L7/L12, in addition to protective role, may show its importance in detection BRUCELLOSIS tests. The aim of this study was to examine antigenicity of recombinant L7/L12 from BRUCELLA ABORTUS by BRUCELLOSIS human sera.Material and Methods: We amplified L7/L12 gene by polymerase chain reaction (PCR) method and sub- cloned to prokaryotic expression vector pET28a. ESCHERICHIA COLI BL21-DE3-plySs were transformed with pET28a-L7/L12 and gene expression was induced by IPTG. Recombinant L7/L12 was further analyzed by WESTERN Blot. Sera reactivity of five infected individual were further analyzed against the recombinant L7/L12 protein.Results: The sequencing result was confirmed by Sanger method and it was the same as L7/L12 gene. ESCHERICHIA COLI BL21 (DE3) pLysS was transformed with pET28a-L7/L12 and gene expression was induced by IPTG. The expressed protein was purified by affinity chromatography by Ni-NTA resin. The data also indicated that L7/L12 protein from BRUCELLA ABORTUS recognized by patient sera.Conclusion: Our data showed that recombinant L7/L12 protein can be produced by pET28a in ESCHERICHIA COLI. This protein was recognized by sera in infected human as an antigen. Therefore, recombinant L7/L12 has same epitopes with natural form of this antigen. Recombinant L7/L12 also seems to be a promising antigen for protection and serologic diagnosis of Human BRUCELLOSIS.

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APA: Copy

ABTAHI, HAMID, & SALMANIAN, ALI HATEF. (2012). ANTIGENICITY OF RECOMBINANT L7/L12 IN PATIENTS WITH BRUCELLOSIS. KOOMESH, 13(2 (42)), 167-171. SID. https://sid.ir/paper/380326/en

Vancouver: Copy

ABTAHI HAMID, SALMANIAN ALI HATEF. ANTIGENICITY OF RECOMBINANT L7/L12 IN PATIENTS WITH BRUCELLOSIS. KOOMESH[Internet]. 2012;13(2 (42)):167-171. Available from: https://sid.ir/paper/380326/en

IEEE: Copy

HAMID ABTAHI, and ALI HATEF SALMANIAN, “ANTIGENICITY OF RECOMBINANT L7/L12 IN PATIENTS WITH BRUCELLOSIS,” KOOMESH, vol. 13, no. 2 (42), pp. 167–171, 2012, [Online]. Available: https://sid.ir/paper/380326/en

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