EXPRESSION OF RECOMBINANT P39 PROTEIN FROM BRUCELLA ABORTUS IN ESCHERICHIA COLI

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ABTAHI H.; SALMANIAN ALI HATEF; BEHZADIANNEZHAD GH.; RAFATI S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Arak University Medical Sciences Year:2004 | Volume:7 | Issue:1 (26) Page(s): 1-7

Download Full-Text

Persian Verion

View:

1,065

Download:

Cites:

Information Journal Paper

Title

EXPRESSION OF RECOMBINANT P39 PROTEIN FROM BRUCELLA ABORTUS IN ESCHERICHIA COLI

Author(s)

ABTAHI H. | SALMANIAN ALI HATEF | BEHZADIANNEZHAD GH. | RAFATI S. | Issue Writer Certificate

Keywords

BRUCELLA ABORTUSQ3

P39 GENEQ4

P39 PROTEINQ4

GENE EXPRESSIONQ2

RECOMBINANTQ4

E. COLIQ3

Abstract

Introduction: Brucellosis, caused by Brucella spp., is an important zoonotic disease that causes abortion and infertility in cattle, and undulant fever in human. At present, live attenuated strain 19 of BRUCELLA ABORTUS is used to immunize domestic animals, but this vaccine has several disadvantages. Therefore, the development of more effective and safer vaccines is necessary for disease control in animals and of more importance in human. So research on development of subunit vaccine seems appropriate, and regarding this, P39 preplasmic protein have shown to be highly immunogenic.Materials and methods: In this experimental study, we amplified P39 GENE by Polymerase chain reaction (PCR) method and sub cloned to prokaryotic expression vector pGEX4T1. Escherichia coli BL21 were transformed with pGEX4T1-P39 and GENE EXPRESSION was induced by IPTG. Then it was purified by GST Sepharose kit. The concentration of P39 was assayed by Bradford method.Results: The RECOMBINANT plasmid (pSK-P39) was sequenced. The sequencing result was confirmed by Sanger method and was same as P39 GENE. The concentration of purified protein was 200 µg/ml.Conclusion: The vectors that contain fusion protein can produce the RECOMBINANT P39 PROTEIN.

Cites

SPECIFICITY OF RECOMBINANT P39 PROTEIN IN DETECTION OF ANTI-BRUCELLA ANTIBODY IN PATIENTS WITH BRUCELLOSIS

References

No record.

Cite

APA: Copy

ABTAHI, H., SALMANIAN, ALI HATEF, BEHZADIANNEZHAD, GH., & RAFATI, S.. (2004). EXPRESSION OF RECOMBINANT P39 PROTEIN FROM BRUCELLA ABORTUS IN ESCHERICHIA COLI. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), 7(1 (26)), 1-7. SID. https://sid.ir/paper/69778/en

Vancouver: Copy

ABTAHI H., SALMANIAN ALI HATEF, BEHZADIANNEZHAD GH., RAFATI S.. EXPRESSION OF RECOMBINANT P39 PROTEIN FROM BRUCELLA ABORTUS IN ESCHERICHIA COLI. ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ)[Internet]. 2004;7(1 (26)):1-7. Available from: https://sid.ir/paper/69778/en

IEEE: Copy

H. ABTAHI, ALI HATEF SALMANIAN, GH. BEHZADIANNEZHAD, and S. RAFATI, “EXPRESSION OF RECOMBINANT P39 PROTEIN FROM BRUCELLA ABORTUS IN ESCHERICHIA COLI,” ARAK MEDICAL UNIVERSITY JOURNAL (AMUJ), vol. 7, no. 1 (26), pp. 1–7, 2004, [Online]. Available: https://sid.ir/paper/69778/en

Related Journal Papers