Investigation of Neuronal Phenotype Induction in P19 Stem Cells Influenced by Extract of Neonatal Rat Brain and Deprenyl

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Momendoust Nazila; MOSHTAGHIAN JAMAL; ESMAEILI FARIBA

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Journal: Jundishapur Scientific Medical Journal Year:2020 | Volume:19 | Issue:1 Page(s): 83-95

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Information Journal Paper

Title

Investigation of Neuronal Phenotype Induction in P19 Stem Cells Influenced by Extract of Neonatal Rat Brain and Deprenyl

Author(s)

Momendoust Nazila | MOSHTAGHIAN JAMAL | ESMAEILI FARIBA | Issue Writer Certificate

Keywords

P19 embryonic carcinoma cellsQ1

DeprenylQ3

Extracts of neonatal rat brainQ1

Neural differentiationQ2

Abstract

Background and Objective: Parkinson's disease is caused by selective loss of a group of dopaminergic cells in the dense part of substantial nigra. One of the treatment options for this disease is the replacement of lost cells by stem cells. The purpose of this study was to investigate the effect of inducers of extract of neonatal rat brain and Deprenyl on Neural differentiation of P19 embryonic carcinoma cells. Materials and Methods: P19 stem cells were cultured in alpha-MEM medium supplemented with 10% FBS serum. Then, the cells were cultured on low-adhesion culture plates to create pseudo-embryonic bodies. In order to differentiate, germ cells were cultured in medium containing 3% FBS serum and extract of neonatal rat brain, Deprenyl and mixture of Deprenyl and rat neonatal extract for 28 days. Immunofluorescence assay was used to detect specific proteins of neurons including synaptophysin, beta-tubulin 3 and nestin. Immunofluorescence assay and Real-time PCR was used to detect and evaluate the expression specific proteins of neurons including synaptophysin, beta-tubulin 3 and nestin, respectively. Results: Regarding to immunofluorescence results, differentiated P19 cells, positively affected by three inducers such as nestin, synaptophysin and beta-3. Relative expression of transcription factors of nestin, synaptophysin in cells differentiated by all three inducer using Real-time PCR demonstrated that in P19 embryonic carcinoma cells, Nestin and Synaptophysin expression increased in the second week, while, their expression were significantly reduced in the third and fourth weeks. Conclusion: The results of this study show P19 embryonic carcinoma stem cells are potential to differentiate into neurons, especially dopaminergic phenotype, following laboratory conditions and inducing factors.

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APA: Copy

Momendoust, Nazila, MOSHTAGHIAN, JAMAL, & ESMAEILI, FARIBA. (2020). Investigation of Neuronal Phenotype Induction in P19 Stem Cells Influenced by Extract of Neonatal Rat Brain and Deprenyl. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, 19(1 ), 83-95. SID. https://sid.ir/paper/383510/en

Vancouver: Copy

Momendoust Nazila, MOSHTAGHIAN JAMAL, ESMAEILI FARIBA. Investigation of Neuronal Phenotype Induction in P19 Stem Cells Influenced by Extract of Neonatal Rat Brain and Deprenyl. JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL[Internet]. 2020;19(1 ):83-95. Available from: https://sid.ir/paper/383510/en

IEEE: Copy

Nazila Momendoust, JAMAL MOSHTAGHIAN, and FARIBA ESMAEILI, “Investigation of Neuronal Phenotype Induction in P19 Stem Cells Influenced by Extract of Neonatal Rat Brain and Deprenyl,” JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, vol. 19, no. 1 , pp. 83–95, 2020, [Online]. Available: https://sid.ir/paper/383510/en

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