THE INDUCTION OF NEURAL PHENOTYPE IN EMBRYONIC STEM (ES) CELLS BY DEPRENYL

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ESMAEILI FARIBA; ALTARIHI T.

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Journal: Daneshvar Medicine: Basic and Clinical Research Journal Year:2007 | Volume:15 | Issue:71 Page(s): 1-10

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Title

THE INDUCTION OF NEURAL PHENOTYPE IN EMBRYONIC STEM (ES) CELLS BY DEPRENYL

Author(s)

ESMAEILI FARIBA | ALTARIHI T. | Issue Writer Certificate

Keywords

EMBRYONIC STEM CELLSQ3

VERO CELL LINEQ2

NEURAL DIFFERENTIATIONQ3

DEPRENYLQ3

CCE CELL LINEQ3

Abstract

Introduction & Objective: Previous studies have shown that ES cells could be induced to differentiate into neurons and gelia in vitro. Induction protocols are based on culture in the presence of an inducer such as RA. In this study, the effect of DEPRENYL on the differentiation of EMBRYONIC STEM CELLS (ES) cells to neuron-like cells was investigated. DEPRENYL is a type B monoamine oxidase inhibitor which has been used for treatment of Parkinson's disease.The drug protects neurons in a variety of modes, by inducing neurotrophin expression, it causes neuritis outgrowth. These raised the possibility of a direct effect of DEPRENYL on pleuripotent ES to develop into neurons.Materials & Methods: Cells of an embryonic stem cell line (CCE) were cultured as aggregates (embryoid bodies) then, they were exposed to a concentration of 10-8M DEPRENYL. CCE was used to evaluate the induction of neuronal differentiation by DEPRENYL. Morphological and immunohistochemical techniques were used to evaluate the differentiation of CCE cells In addition to the Cresyl violet used for morphological study, antinestin antibody was applied to characterize the neuroepithelial differentiation. Moreover, anti-synaptophysin and anti-tyrosine hydroxylase antibodies were used to characterize the neuronal phenotype of ESCs.Results: The results indicated that the concentration of 10-8M DEPRENYL could induce the differentiation of ESCs into neurons.

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APA: Copy

ESMAEILI, FARIBA, & ALTARIHI, T.. (2007). THE INDUCTION OF NEURAL PHENOTYPE IN EMBRYONIC STEM (ES) CELLS BY DEPRENYL. DANESHVAR MEDICINE, 15(71), 1-10. SID. https://sid.ir/paper/30413/en

Vancouver: Copy

ESMAEILI FARIBA, ALTARIHI T.. THE INDUCTION OF NEURAL PHENOTYPE IN EMBRYONIC STEM (ES) CELLS BY DEPRENYL. DANESHVAR MEDICINE[Internet]. 2007;15(71):1-10. Available from: https://sid.ir/paper/30413/en

IEEE: Copy

FARIBA ESMAEILI, and T. ALTARIHI, “THE INDUCTION OF NEURAL PHENOTYPE IN EMBRYONIC STEM (ES) CELLS BY DEPRENYL,” DANESHVAR MEDICINE, vol. 15, no. 71, pp. 1–10, 2007, [Online]. Available: https://sid.ir/paper/30413/en

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