Determination of malachite green and leucomalachite green residues in rainbow trout in Sanandaj City

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REZAEE REZA; MALEKI AFSHIN; SAFARI MAHDI; SHAHMORADI BEHZAD; JAFARI ALI; GIAHI OMID; ZANDI SHIVA; Khaledyan Nikoo; SHARIFI SHIMA; Mohammadi Shadieh

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Journal: Scientific Journal of Kurdistan University of Medical Sciences Year:2020 | Volume:25 | Issue:3 (107) Page(s): 61-71

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Title

Determination of malachite green and leucomalachite green residues in rainbow trout in Sanandaj City

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Keywords

Malachite greenQ1

Leucomalachite greenQ1

Rainbow troutQ1

ELISAQ2

SanandajQ2

Abstract

Background and Aim: Malachite green (MG) is an organic compound which his used as a dye in textile industry. This compound is applied incorrectly and illegally in aquiculture as an antimicrobial agent. This compound has mutagenic and carcinogenic effects. Materials and Methods: In this study we determined the concentrations of MG and leucoMalachite green (LMG) in the muscle tissue of 90 Rainbow trouts (Oncorhynchus mykiss) collected from the local markets in Sanandaj City within a period of six months in 2018. In order to measure the residues of MG and LMG, we used enzyme-linked immunosorbent assay (ELISA) based on the European Union Decision 2002/657/EC. Results: The results showed presence of MG and LMG in all 90 samples: The residues of MG in the samples were as follows: 1-2 μ g/Kg in 41 (6. 45%), more than 2 μ g/Kg in 45 (50%) and less than 1 μ g/Kg in 4 samples (4. 4%). Furthermore, the residues of LMG in the samples were as follows: more than 2 μ g/Kg in 13 (45. 14%), 1-2 μ g/Kg in 67 (44. 74%) and less than 1 μ g/Kg in 10 samples (11. 11%). Moreover, the limit of detection (LOD) in the method was 0. 43 μ g/Kg. Conclusions: It is concluded that MG is illegally applied in fish farms, which can be a threat to human health and the environment.

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APA: Copy

REZAEE, REZA, MALEKI, AFSHIN, SAFARI, MAHDI, SHAHMORADI, BEHZAD, JAFARI, ALI, GIAHI, OMID, ZANDI, SHIVA, Khaledyan, Nikoo, SHARIFI, SHIMA, & Mohammadi, Shadieh. (2020). Determination of malachite green and leucomalachite green residues in rainbow trout in Sanandaj City. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, 25(3 (107) ), 61-71. SID. https://sid.ir/paper/383996/en

Vancouver: Copy

REZAEE REZA, MALEKI AFSHIN, SAFARI MAHDI, SHAHMORADI BEHZAD, JAFARI ALI, GIAHI OMID, ZANDI SHIVA, Khaledyan Nikoo, SHARIFI SHIMA, Mohammadi Shadieh. Determination of malachite green and leucomalachite green residues in rainbow trout in Sanandaj City. SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2020;25(3 (107) ):61-71. Available from: https://sid.ir/paper/383996/en

IEEE: Copy

REZA REZAEE, AFSHIN MALEKI, MAHDI SAFARI, BEHZAD SHAHMORADI, ALI JAFARI, OMID GIAHI, SHIVA ZANDI, Nikoo Khaledyan, SHIMA SHARIFI, and Shadieh Mohammadi, “Determination of malachite green and leucomalachite green residues in rainbow trout in Sanandaj City,” SCIENTIFIC JOURNAL OF KURDISTAN UNIVERSITY OF MEDICAL SCIENCES, vol. 25, no. 3 (107) , pp. 61–71, 2020, [Online]. Available: https://sid.ir/paper/383996/en

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