ISOLATION OF SKIN-DERIVED PRECURSORS FROM HUMAN FORESKIN AND THEIR DIFFERENTIATION INTO NEURONS AND GLIAL CELLS

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BAKHTIARI MITRA; MANSOURI KAMRAN; MOSTAFAIE ALI; SADEGHI YOUSEF; MOZAFARI HADI; GHORBANI ROSTAM; REZAEI TAVIRANI MOSTAFA

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Journal: Tehran University Medical Journal Year:2010 | Volume:68 | Issue:9 Page(s): 508-515

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Title

ISOLATION OF SKIN-DERIVED PRECURSORS FROM HUMAN FORESKIN AND THEIR DIFFERENTIATION INTO NEURONS AND GLIAL CELLS

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Keywords

SKIN-DERIVED PRECURSORQ3

NEURAL LINEAGEQ3

NEURONQ3

GLIAL CELLQ3

DIFFERENTIATIONQ3

Abstract

Background: SKIN-DERIVED PRECURSORs (SKPs) are a type of progenitor cells extracted from mammalian dermal tissue and can be differentiate to neural and mesodermal lineage in vitro. These cells can introduce an accessible autologos source of neural precursor cells for treatment of different neurodegenerative diseases. This research was done in order to set up isolation, culture, proliferation and DIFFERENTIATION of human skin derived precursors (hSKPs).Methods: Human foreskin samples were cut into smaller pieces and cultured in proliferation medium after enzymatic digestion. To induce neural DIFFERENTIATION, cells were cultured in neural DIFFERENTIATION medium after fifth passage. We used immunocytochemistry and RT-PCR for characterization of the cells. NEURON and GLIAL CELL DIFFERENTIATION potential was assessed by immunofloresence using specific antibodies. The experiments were carried out in triplicate.Results: After DIFFERENTIATION, bΙΙΙ- tubulin and neurofilament-M positive cells were observed that are specific markers for NEURONs. Moreover, glial fibrillary acid protein (GFAP) and S100 positive cells were identified that are markers specifically express in GLIAL CELLs. Detected NEURONs and glials were also confirmed by their morphologic characterizations.Conclusion: Our results demonstrated that SKIN-DERIVED PRECURSORs obtained from human foreskin can exhibit NEURONal and glial DIFFERENTIATION potential in vitro, depending on the protocols of induction.

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APA: Copy

BAKHTIARI, MITRA, MANSOURI, KAMRAN, MOSTAFAIE, ALI, SADEGHI, YOUSEF, MOZAFARI, HADI, GHORBANI, ROSTAM, & REZAEI TAVIRANI, MOSTAFA. (2010). ISOLATION OF SKIN-DERIVED PRECURSORS FROM HUMAN FORESKIN AND THEIR DIFFERENTIATION INTO NEURONS AND GLIAL CELLS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), 68(9), 508-515. SID. https://sid.ir/paper/39557/en

Vancouver: Copy

BAKHTIARI MITRA, MANSOURI KAMRAN, MOSTAFAIE ALI, SADEGHI YOUSEF, MOZAFARI HADI, GHORBANI ROSTAM, REZAEI TAVIRANI MOSTAFA. ISOLATION OF SKIN-DERIVED PRECURSORS FROM HUMAN FORESKIN AND THEIR DIFFERENTIATION INTO NEURONS AND GLIAL CELLS. TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ)[Internet]. 2010;68(9):508-515. Available from: https://sid.ir/paper/39557/en

IEEE: Copy

MITRA BAKHTIARI, KAMRAN MANSOURI, ALI MOSTAFAIE, YOUSEF SADEGHI, HADI MOZAFARI, ROSTAM GHORBANI, and MOSTAFA REZAEI TAVIRANI, “ISOLATION OF SKIN-DERIVED PRECURSORS FROM HUMAN FORESKIN AND THEIR DIFFERENTIATION INTO NEURONS AND GLIAL CELLS,” TEHRAN UNIVERSITY MEDICAL JOURNAL (TUMJ), vol. 68, no. 9, pp. 508–515, 2010, [Online]. Available: https://sid.ir/paper/39557/en

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