DIAGNOSIS OF LIVER AND PULMONARY HYDATID CYST BY DOT-ELISA, IFA AND IHA TESTS

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GHORBANIKHANY D.; DAILAMI A.

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Journal: Feyz Medical Sciences Journal Year:2000 | Volume:4 | Issue:13 Page(s): 36-42

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Title

DIAGNOSIS OF LIVER AND PULMONARY HYDATID CYST BY DOT-ELISA, IFA AND IHA TESTS

Author(s)

GHORBANIKHANY D. | DAILAMI A. | Issue Writer Certificate

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Abstract

History and Objectives: Although some serological tests are not highly sensitive in diagnosis of pulmonary hydatide cysts however Dot-ELISA is introduced as a simple, sensitive and a very efficient means of pulmonary hydatid cyst diagnosis. In the present study, we compared the validity of IHA, IFA and Dot-ELISA techniques in diagnosis of antiechinococcal antibodies caused bu Echinococcus granulosis in cystic liver and lung hydatid disease. Materials and Methods: 244 sera of which 30 samples from hydatid patients, 104 sera from patients suffering a disease other than hydatidosis and 110 serafrom healthy individuals were collected mainly from Tehran's hospitals. The hydatid patients were confirmed by surgery and consisted of 14, 10 6patients with liver, lung and liver-lung hydatid cysts respectively. The sera were tested by IHA, IFA and Dot-ELISA techniques. Sensitivity, spedficity, predictive values, false positivity and false negativity of each test were evaluated in diagnosis of liver and lung hydatid cysts.Results: The results indicate that the sensitivities of IHA test for liver, lung and liver-lung cysts were 78.7%, 60% and 100% respectively, while the sensitivities of IFA in detecting liver, lung and liver-lung cysts were 93%, 80% and 100% respectively. In this regard, the Dot-ELISA showed the best results whereas its sensitivities for liver, lung and liver-lung cysts were 100% each.Conclusions: These results indicates that detection of antiechinococcal antibodies in human serais superior by Dot-ELISA compared to IHA and IFA tests. Hence, the application of this test in medical laboratories for hydatid cyst diagnosis can be recommended.

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کاربرد روش ایمونوفلورسانس در تشخیص کیست هیداتیک و مقایسه آن با روش های کلاسیک در تهران

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APA: Copy

GHORBANIKHANY, D., & DAILAMI, A.. (2000). DIAGNOSIS OF LIVER AND PULMONARY HYDATID CYST BY DOT-ELISA, IFA AND IHA TESTS . FEYZ, 4(13), 36-42. SID. https://sid.ir/paper/40714/en

Vancouver: Copy

GHORBANIKHANY D., DAILAMI A.. DIAGNOSIS OF LIVER AND PULMONARY HYDATID CYST BY DOT-ELISA, IFA AND IHA TESTS . FEYZ[Internet]. 2000;4(13):36-42. Available from: https://sid.ir/paper/40714/en

IEEE: Copy

D. GHORBANIKHANY, and A. DAILAMI, “DIAGNOSIS OF LIVER AND PULMONARY HYDATID CYST BY DOT-ELISA, IFA AND IHA TESTS ,” FEYZ, vol. 4, no. 13, pp. 36–42, 2000, [Online]. Available: https://sid.ir/paper/40714/en

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