PRODUCTION OF CAMEL POLYCLONAL ANTIBODY AGAINST VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2 AND PERFORMANCE EVALUATION IN ELISA TEST

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BEHDANI MAHDI; KARIMIPOUR MORTEZA; KHANAHMAD SHAHREZA HOSSEIN; ASADZADEH NADER; AZADMANESH KEYHAN; KHABIRI ALIREZA; HABIBI ANBOHI MEHDI; ZEINALI SIROUS

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Journal: Research in Medicine Year:2011 | Volume:35 | Issue:2 Page(s): 93-98

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Title

PRODUCTION OF CAMEL POLYCLONAL ANTIBODY AGAINST VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2 AND PERFORMANCE EVALUATION IN ELISA TEST

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Keywords

CAMELQ3

SINGLE CHAIN ANTIBODYQ2

POLYCLONAL ANTIBODYQ2

VEGFR-2Q2

Abstract

Background: In molecular approach, serum of CAMEL contains a unique type of antibodies devoid of light chains since the light chain is missing, the heavy-chain antibodies should bind their antigen by one single domain, the variable domain of the heavy immunoglobulin chain. Vascular endothelial growth factor receptor-2 (VEGFR-2) is one of the important proteins in angiogenesis which over-expressed in many types of tumors.Methods: Two male dromedaries (CAMELus dromedarius) were immunized against VEGFR-2. ELISA was used to evaluate the immunization process. CAMEL immune sera were fractionated with protein A and G affinity chromatography. Heavy chain antibodies tested for its specific reactivity in cell-based ELISA in recognition of VEGFR-2 on the cell surface of three cell lines; 293/KDR, HUVECs and A431.Results: In ELISA test, the CAMEL sera in 1/12800 dilution had the acceptable results. Furthermore, cell-based ELISA demonstrated that the polyclonal heavy chain antibodies bind to VEGFR-2 in 293/KDR and HUVECs cell linesConclusion: Single chain polyclonal antibodies against VEGFR-2 can be used for detection of soluble form of this protein by ELISA, and also flowcytometry, western blotting and immunohistochemistry.

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APA: Copy

BEHDANI, MAHDI, KARIMIPOUR, MORTEZA, KHANAHMAD SHAHREZA, HOSSEIN, ASADZADEH, NADER, AZADMANESH, KEYHAN, KHABIRI, ALIREZA, HABIBI ANBOHI, MEHDI, & ZEINALI, SIROUS. (2011). PRODUCTION OF CAMEL POLYCLONAL ANTIBODY AGAINST VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2 AND PERFORMANCE EVALUATION IN ELISA TEST. PAJOUHESH DAR PEZESHKI, 35(2), 93-98. SID. https://sid.ir/paper/41715/en

Vancouver: Copy

BEHDANI MAHDI, KARIMIPOUR MORTEZA, KHANAHMAD SHAHREZA HOSSEIN, ASADZADEH NADER, AZADMANESH KEYHAN, KHABIRI ALIREZA, HABIBI ANBOHI MEHDI, ZEINALI SIROUS. PRODUCTION OF CAMEL POLYCLONAL ANTIBODY AGAINST VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2 AND PERFORMANCE EVALUATION IN ELISA TEST. PAJOUHESH DAR PEZESHKI[Internet]. 2011;35(2):93-98. Available from: https://sid.ir/paper/41715/en

IEEE: Copy

MAHDI BEHDANI, MORTEZA KARIMIPOUR, HOSSEIN KHANAHMAD SHAHREZA, NADER ASADZADEH, KEYHAN AZADMANESH, ALIREZA KHABIRI, MEHDI HABIBI ANBOHI, and SIROUS ZEINALI, “PRODUCTION OF CAMEL POLYCLONAL ANTIBODY AGAINST VASCULAR ENDOTHELIAL GROWTH FACTOR RECEPTOR-2 AND PERFORMANCE EVALUATION IN ELISA TEST,” PAJOUHESH DAR PEZESHKI, vol. 35, no. 2, pp. 93–98, 2011, [Online]. Available: https://sid.ir/paper/41715/en

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