PRODUCTION OF POLYCLONAL ANTIBODY AGAINST RECOMBINANT GROWTH HORMONE AND DESIGNING AN ELISA KIT AND COMPARING SOME OF ITS DIAGNOSTICS INDICES WITH A COMMERCIAL KIT

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MANEIAN MOSTAFA; ZARKESH ESFAHANI SAYYED HAMID; AKBARI MOJTABA; KHANAHMAD HOSSEIN; MASJEDI MOHSEN

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Journal: Journal of Isfahan Medical School Year:2013 | Volume:31 | Issue:247 Page(s): 1173-1184

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Title

PRODUCTION OF POLYCLONAL ANTIBODY AGAINST RECOMBINANT GROWTH HORMONE AND DESIGNING AN ELISA KIT AND COMPARING SOME OF ITS DIAGNOSTICS INDICES WITH A COMMERCIAL KIT

Author(s)

Keywords

TOXOPLASMA GONDIIQ2

TOXOPLASMOSISQ2

IGMQ3

IGGQ3

RISK FACTORQ3

Abstract

Background: TOXOPLASMOSIS is caused by an intracellular protozoan, TOXOPLASMA GONDII. In pregnant women with TOXOPLASMOSIS, the parasite can be transmitted to the fetus, causing severe complications. The aim of this study was to determine the seroprevalence and RISK FACTORs of TOXOPLASMOSIS in women of reproductive age in Tehran, Iran, to provide detailed information for health authorities regarding the prevention and control of the disease.Methods: In this cross-sectional study conducted in 2013, completion of the questionnaires was done and blood samples were collected from 300 women of 18-40 years of age. Levels of IGG and IGM antibodies against TOXOPLASMA GONDII were determined by enzyme-linked immunosorbent assay (ELISA) test and the RISK FACTORs cited in questionnaires were also analyzed.Findings: Among 300 studied sera, 85 (28.3%) cases were positive for IGG, 4 (1.3%) for IGM and 3 (1%) for both classes. There were no significant relationship between TOXOPLASMOSIS and factors such as employment, education, eating raw meat and vegetables and contact with cats (chi-square test); but there was a significant relationship between TOXOPLASMOSIS and age (Mann-Whitney test, P = 0.04).Conclusion: 70.4 percent of selected women in the study were seronegative. It is necessary to avoid the RISK FACTORs during and before the pregnancy for women via health education.

Cites

PURIFICATION OF INFLUENZA VIRUS A (H1N1) RECOMBINANT HEMAGGLUTININ (HA1) AND POLYCLONAL ANTIBODY PRODUCTION

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APA: Copy

MANEIAN, MOSTAFA, ZARKESH ESFAHANI, SAYYED HAMID, AKBARI, MOJTABA, KHANAHMAD, HOSSEIN, & MASJEDI, MOHSEN. (2013). PRODUCTION OF POLYCLONAL ANTIBODY AGAINST RECOMBINANT GROWTH HORMONE AND DESIGNING AN ELISA KIT AND COMPARING SOME OF ITS DIAGNOSTICS INDICES WITH A COMMERCIAL KIT. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), 31(247), 1173-1184. SID. https://sid.ir/paper/49849/en

Vancouver: Copy

MANEIAN MOSTAFA, ZARKESH ESFAHANI SAYYED HAMID, AKBARI MOJTABA, KHANAHMAD HOSSEIN, MASJEDI MOHSEN. PRODUCTION OF POLYCLONAL ANTIBODY AGAINST RECOMBINANT GROWTH HORMONE AND DESIGNING AN ELISA KIT AND COMPARING SOME OF ITS DIAGNOSTICS INDICES WITH A COMMERCIAL KIT. JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S)[Internet]. 2013;31(247):1173-1184. Available from: https://sid.ir/paper/49849/en

IEEE: Copy

MOSTAFA MANEIAN, SAYYED HAMID ZARKESH ESFAHANI, MOJTABA AKBARI, HOSSEIN KHANAHMAD, and MOHSEN MASJEDI, “PRODUCTION OF POLYCLONAL ANTIBODY AGAINST RECOMBINANT GROWTH HORMONE AND DESIGNING AN ELISA KIT AND COMPARING SOME OF ITS DIAGNOSTICS INDICES WITH A COMMERCIAL KIT,” JOURNAL OF ISFAHAN MEDICAL SCHOOL (I.U.M.S), vol. 31, no. 247, pp. 1173–1184, 2013, [Online]. Available: https://sid.ir/paper/49849/en

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