POLYMERASE CHAIN REACTION VERSUS CONVENTIONAL LABORATORY METHODS IN THE DIAGNOSIS OF FUNGAL KERATITIS

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NEJABAT MAHMOUD; KHALILI MOHAMMAD REZA; BADIEI PARISA; KESHAVARZ FAZL F.; ALBORZI A.A.V.; SHADMANI ATHAR

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Journal: BINA Year:2009 | Volume:15 | Issue:1 (58) Page(s): 63-67

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Title

POLYMERASE CHAIN REACTION VERSUS CONVENTIONAL LABORATORY METHODS IN THE DIAGNOSIS OF FUNGAL KERATITIS

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Keywords

POLYMERASE CHAIN REACTIONQ3

KERATITISQ3

EYE INFECTIONSQ3

FUNGALQ3

Abstract

Purpose: To compare the value of conventional laboratory methods and POLYMERASE CHAIN REACTION (PCR)in the diagnosis of FUNGAL KERATITIS.Methods: This cross sectional study was conducted at Khalili Hospital, Shiraz, Iran. Samples were taken from thirty-eight patients with findings suspicious for FUNGAL KERATITIS. Corneal scrapings were used for Gram, Giemsa and KOH stains, culture and PCR analysis.Results: Of 38 enrolled eyes, 25 eyes (68.5%) were judged to have FUNGAL infection based on positive cultures, staining, PCR or response to antifungal treatment. PCR detected fungi DNA in 17 of 25 samples (68% sensitivity). Staining (Gram, Giemsa and KOH) and culture yielded a positive result in 40% and 24% of samples respectively. Twenty one (84%) of 25 patients showed FUNGAL elements in at least one laboratory work up and 4 patients were diagnosed as FUNGAL KERATITIS only based on response to antifungal drugs. Conclusion: Compared to conventional laboratory methods, PCR based methods offer higher sensitivity and a faster diagnosis in FUNGAL KERATITIS.

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APA: Copy

NEJABAT, MAHMOUD, KHALILI, MOHAMMAD REZA, BADIEI, PARISA, KESHAVARZ FAZL, F., ALBORZI, A.A.V., & SHADMANI, ATHAR. (2009). POLYMERASE CHAIN REACTION VERSUS CONVENTIONAL LABORATORY METHODS IN THE DIAGNOSIS OF FUNGAL KERATITIS. BINA, 15(1 (58)), 63-67. SID. https://sid.ir/paper/42240/en

Vancouver: Copy

NEJABAT MAHMOUD, KHALILI MOHAMMAD REZA, BADIEI PARISA, KESHAVARZ FAZL F., ALBORZI A.A.V., SHADMANI ATHAR. POLYMERASE CHAIN REACTION VERSUS CONVENTIONAL LABORATORY METHODS IN THE DIAGNOSIS OF FUNGAL KERATITIS. BINA[Internet]. 2009;15(1 (58)):63-67. Available from: https://sid.ir/paper/42240/en

IEEE: Copy

MAHMOUD NEJABAT, MOHAMMAD REZA KHALILI, PARISA BADIEI, F. KESHAVARZ FAZL, A.A.V. ALBORZI, and ATHAR SHADMANI, “POLYMERASE CHAIN REACTION VERSUS CONVENTIONAL LABORATORY METHODS IN THE DIAGNOSIS OF FUNGAL KERATITIS,” BINA, vol. 15, no. 1 (58), pp. 63–67, 2009, [Online]. Available: https://sid.ir/paper/42240/en

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