PRODUCTION OF CONJUGATED LINOLEIC ACID BY TRANSFORMED E.COLI

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FARMANI JAMSHID; SAFARI MOHAMMAD; ROOHVAND FARZIN; AGHASADEGHI MOHAMMAD REZA; RAZAVI SEYED HADI; MOTEVALLI FATEMEH

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Journal: Journal of Mazandaran University of Medical Sciences Year:2011 | Volume:21 | Issue:82 Page(s): 37-46

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Title

PRODUCTION OF CONJUGATED LINOLEIC ACID BY TRANSFORMED E.COLI

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Keywords

CONJUGATED LINOLEIC ACID (CLA)Q2

LINOLEIC ACID ISOMERASEQ2

E. COLIQ2

PROPIONIBACTERIUM ACNESQ2

Abstract

Background and purpose: Due to the beneficial physiological effects of conjugated linoleic acid (CLA), there has been a growing tendency to produce it as a functional lipid in recent years. Different CLA isomers have different physiological effects; hence, production of certain CLA isomers with high purity is of great importance. CLA can be produced through both chemical and enzymatic methods; however, unlike chemical catalysts, enzymes make it possible to produce pure CLA isomers. In this study, LINOLEIC ACID ISOMERASE from PROPIONIBACTERIUM ACNES was expressed in E. COLI and the possibility of the production of CLA was studied.Materials and methods: The vector containing LINOLEIC ACID ISOMERASE, pGEX-6P-PAI, was transformed inE. coli. Transformants were selected based on their resistance to ampicillin and restriction digestion analysis. To express the recombinant LINOLEIC ACID ISOMERASE, transformants were induced using isopropyl-beta-thiogalactopyranoside (IPTG). The expression of recombinant protein was confirmed by SDS-polyacrylamide gel electrophoresis and Western blotting using anti-linoleic acid isomerase antibody. Then, the possibility of the production of CLA from Linoleic acid by usingE-coli transformant was investigated.Results: Recombinant LINOLEIC ACID ISOMERASE was intracellularly produced as a glutathione Stransferase (GST) tagged protein by transformedE-coli. The fusion of GST to the N-terminus of LINOLEIC ACID ISOMERASE increased its molecular weight from 49 to 75 kDa. GST-tagged enzyme acted like LINOLEIC ACID ISOMERASE and the transformed bacterium could convert considerable amounts of linoleic acid to CLA.Conclusion: The findings indicated that transformed E. COLI can be used for CLA production in biocatalytic processes.

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APA: Copy

FARMANI, JAMSHID, SAFARI, MOHAMMAD, ROOHVAND, FARZIN, AGHASADEGHI, MOHAMMAD REZA, RAZAVI, SEYED HADI, & MOTEVALLI, FATEMEH. (2011). PRODUCTION OF CONJUGATED LINOLEIC ACID BY TRANSFORMED E.COLI. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 21(82), 37-46. SID. https://sid.ir/paper/44539/en

Vancouver: Copy

FARMANI JAMSHID, SAFARI MOHAMMAD, ROOHVAND FARZIN, AGHASADEGHI MOHAMMAD REZA, RAZAVI SEYED HADI, MOTEVALLI FATEMEH. PRODUCTION OF CONJUGATED LINOLEIC ACID BY TRANSFORMED E.COLI. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2011;21(82):37-46. Available from: https://sid.ir/paper/44539/en

IEEE: Copy

JAMSHID FARMANI, MOHAMMAD SAFARI, FARZIN ROOHVAND, MOHAMMAD REZA AGHASADEGHI, SEYED HADI RAZAVI, and FATEMEH MOTEVALLI, “PRODUCTION OF CONJUGATED LINOLEIC ACID BY TRANSFORMED E.COLI,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 21, no. 82, pp. 37–46, 2011, [Online]. Available: https://sid.ir/paper/44539/en

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