IMPROVED ACANTHAMOEBA CULTURE METHOD USING TYM MEDIUM

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Ziaei Hezarjaribi Hajar; NAYERI CHEGENI TOORAN; DADIMOGHADAM YOUSEF; KHOSHZABAN FARIBA; GHAFFARIFAR FATEMEH; FAKHAR MAHDI

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Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2018 | Volume:28 | Issue:160 Page(s): 156-160

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Title

IMPROVED ACANTHAMOEBA CULTURE METHOD USING TYM MEDIUM

Author(s)

Keywords

ACANTHAMOEBAQ1

TROPHOZOITEQ1

NNA MEDIUMQ1

TYM MEDIUMQ1

Abstract

Background and purpose: ACANTHAMOEBA species are ubiquitous amphizoic organisms whichcan cause lethal diseases, such as keratitis and encephalitis in domestic animals and humans. The firststage in studies related to ACANTHAMOEBA is achieving abundant amount of amoebae in culture medium. The aim of this study was to evaluate TYM MEDIUM as a rich medium for the diagnosis of ACANTHAMOEBAkeratitis in corneal scrapes. Materials and Methods: In this experimental study, we used one species of ACANTHAMOEBA thatwas previously genotyped. ACANTHAMOEBA was cultured in five plates of non-nutrient agar (NNA)medium with E. coli without TYM and in five plates of non-nutrient agar containing E. coli and TYM. Amoebae growth was observed for 24 to 48 hours by invert microscope. Results: According to current results using TYM MEDIUM increased the growth of TROPHOZOITEs8. 8 folds and amoebas can be isolated form medium after 24 to 48 hours. Conclusion: Due to the significant growth of ACANTHAMOEBA in NNA improved medium, it isrecommended for agglutination and early detection of ACANTHAMOEBA in clinical and environmentalsamples.

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APA: Copy

Ziaei Hezarjaribi, Hajar, NAYERI CHEGENI, TOORAN, DADIMOGHADAM, YOUSEF, KHOSHZABAN, FARIBA, GHAFFARIFAR, FATEMEH, & FAKHAR, MAHDI. (2018). IMPROVED ACANTHAMOEBA CULTURE METHOD USING TYM MEDIUM. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 28(160 ), 156-160. SID. https://sid.ir/paper/45202/en

Vancouver: Copy

Ziaei Hezarjaribi Hajar, NAYERI CHEGENI TOORAN, DADIMOGHADAM YOUSEF, KHOSHZABAN FARIBA, GHAFFARIFAR FATEMEH, FAKHAR MAHDI. IMPROVED ACANTHAMOEBA CULTURE METHOD USING TYM MEDIUM. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2018;28(160 ):156-160. Available from: https://sid.ir/paper/45202/en

IEEE: Copy

Hajar Ziaei Hezarjaribi, TOORAN NAYERI CHEGENI, YOUSEF DADIMOGHADAM, FARIBA KHOSHZABAN, FATEMEH GHAFFARIFAR, and MAHDI FAKHAR, “IMPROVED ACANTHAMOEBA CULTURE METHOD USING TYM MEDIUM,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 28, no. 160 , pp. 156–160, 2018, [Online]. Available: https://sid.ir/paper/45202/en

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