Inhibitory Effect of Coadministration of Silibinin and CpGODN2006 on Metastatic Characteristics (MMP-2 and TLR-9) of Hepatocarcinoma Cell Line

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

REZAEI AREZOU; SAADAT FARSHID; SAFAVIFAR FARNAZ; BERAHMEH AZAR; KHORRAMIZADEH MOHAMMAD REZA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES Year:2019 | Volume:29 | Issue:171 Page(s): 1-10

Download Full-Text

Persian Verion

View:

496

Download:

Cites:

Information Journal Paper

Title

Inhibitory Effect of Coadministration of Silibinin and CpGODN2006 on Metastatic Characteristics (MMP-2 and TLR-9) of Hepatocarcinoma Cell Line

Author(s)

Keywords

silibininQ1

CpG-oligodeoxynucleotidesQ1

MMP2Q1

TLR9Q1

HepG-2Q1

Abstract

Background and purpose: Hepatocellular carcinoma (HCC) is a most common liver malignancy and Toll-like receptor-9 (TLR9) is essential for CpG DNA-induced immune responses. The aim of this study was to assess the anti-metastatic effects of combined administration of silibinin and CpG-ODN2006 on human hepatocellular carcinoma HepG-2 cell line model. Materials and methods: Inhibitory effects of various concentrations of silibinin and CpGODN2006 on HepG-2 cells growth and detection of best dosages for simultaneous usage were assessed by Methylthiazolyldiphenyl-tetrazolium bromide (MTT) method. Expression levels of Matrix Metalloproteinase-2 (MMP2) and TLR9 genes were analyzed by real-time PCR. Gelatin zymography was used to determine the activity of MMP2. Results: Inhibitory effect of coadministration of silibinin (100 μ M) and CpG-ODN2006 (100 nM) on HepG-2 cells growth was significant (P<0. 05). Real-Time PCR showed no significant differences in cycle threshold of the genes expression analyses (P>0. 05). Conclusion: Low dose CpG administration efficiently customized silibinin impact on hepatic cancer. Low doses of the drugs and activating immune mechnasims are definitely preferred, therefore, the co-adminstration of these drugs provide a clear vision for the future of combination therapy.

Cites

No record.

References

Cite

APA: Copy

REZAEI, AREZOU, SAADAT, FARSHID, SAFAVIFAR, FARNAZ, BERAHMEH, AZAR, & KHORRAMIZADEH, MOHAMMAD REZA. (2019). Inhibitory Effect of Coadministration of Silibinin and CpGODN2006 on Metastatic Characteristics (MMP-2 and TLR-9) of Hepatocarcinoma Cell Line. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, 29(171 ), 1-10. SID. https://sid.ir/paper/45483/en

Vancouver: Copy

REZAEI AREZOU, SAADAT FARSHID, SAFAVIFAR FARNAZ, BERAHMEH AZAR, KHORRAMIZADEH MOHAMMAD REZA. Inhibitory Effect of Coadministration of Silibinin and CpGODN2006 on Metastatic Characteristics (MMP-2 and TLR-9) of Hepatocarcinoma Cell Line. JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2019;29(171 ):1-10. Available from: https://sid.ir/paper/45483/en

IEEE: Copy

AREZOU REZAEI, FARSHID SAADAT, FARNAZ SAFAVIFAR, AZAR BERAHMEH, and MOHAMMAD REZA KHORRAMIZADEH, “Inhibitory Effect of Coadministration of Silibinin and CpGODN2006 on Metastatic Characteristics (MMP-2 and TLR-9) of Hepatocarcinoma Cell Line,” JOURNAL OF MAZANDARAN UNIVERSITY OF MEDICAL SCIENCES, vol. 29, no. 171 , pp. 1–10, 2019, [Online]. Available: https://sid.ir/paper/45483/en

Related Journal Papers